Document Detail

Inhibition of adenosine 3':k'-monophosphate accumulation white fat acids, lactate, and beta-hydroxybutyrate.
MedLine Citation:
PMID:  181503     Owner:  NLM     Status:  MEDLINE    
The large increase in cyclic AMP accumulation by rat white fat cells seen in the presence of lipolytic agents plus methylxanthines and adenosine deaminase was markedly inhibited by lactate. However, lipolysis was unaffected by lactate. Octanoate, hexanoate, heptanoate, and beta-hydroxybutyrate inhibited both cyclic AMP accumulation and lipolysis by rat fat cells. The mechanism by which these acids inhibit lipolysis differs from that for long chain fatty acids such as oleate. Oleate directly inhibited triglyceride lipase activity of homogenized rat adipose tissue. In contrast, octanoate, beta-hydroxybutyrate, and lacatate had no effect on triglyceride lipase activity. Hormone-stimulated adenylate cyclase activity of rat fat cell ghosts was inhibited by oleate and 4mM octanoate but not by 1.6 mM octanoate, heptanoate, hexanoate, beta-hydroxybutyrate or lactate. None of the acids affected the soluble protein kinase activity of rat adipose tissue. There was no stimulation by lactate, butyrate, beta-hydroxybutyrate, or octanoate of the soluble or particulate cyclic AMP antilipolytic action of a short chain acid such as octanoate or hexanoate was not accompanied by any drop in total fat cell ATP. The mechanism by which lactate lowers cyclic AMP but not lipolysis remains to be established.
J N Fain; R E Shephard
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Journal of lipid research     Volume:  17     ISSN:  0022-2275     ISO Abbreviation:  J. Lipid Res.     Publication Date:  1976 Jul 
Date Detail:
Created Date:  1976-10-02     Completed Date:  1976-10-02     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0376606     Medline TA:  J Lipid Res     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  377-85     Citation Subset:  IM    
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MeSH Terms
Adenylate Cyclase / metabolism
Adipose Tissue / drug effects,  metabolism*
Cyclic AMP / metabolism*
Enzyme Activation / drug effects
Fatty Acids, Nonesterified / pharmacology*
Hydroxybutyrates / pharmacology*
Lactates / pharmacology*
Lipid Mobilization / drug effects
Norepinephrine / pharmacology
Species Specificity
Structure-Activity Relationship
Theophylline / pharmacology
Reg. No./Substance:
0/Fatty Acids, Nonesterified; 0/Hydroxybutyrates; 0/Lactates; 51-41-2/Norepinephrine; 58-55-9/Theophylline; 60-92-4/Cyclic AMP; EC Cyclase

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