| Inhibition of active sodium transport by cytochalasin B in rat jejunum in vitro. | |
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MedLine Citation:
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PMID: 6307390 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The effects of cytochalasin B on electrophysiological properties and sodium transport in rat jejunum in vitro are described. Stripped paired rat jejunal segments were maintained in Ussing chambers with Leibovitz's (L-15) tissue culture medium bubbled with 100% oxygen. L-15 medium contains galactose as the only sugar, and an assortment of amino acids and cofactors to nourish the tissue. Electrophysiological parameters of short-circuit current (Isc) and transepithelial potential difference could be maintained for up to 4 h in control tissues. Upon application of cytochalasin B (20 micrograms/ml), on the mucosal side, Isc and potential difference fell within 1 h from 1.93 +/- 0.12 to 1.09 +/- 0.14 (mean +/- S.E.) muequiv./cm2 per h and from 5 to 2.5 mV. Tissue resistance remained unchanged at approx. 110 omega X cm2 for up to 4 h. 22Na net flux was 4.1 +/- 0.9 muequiv./cm2 per h during the last control period and fell to zero within 1 h after cytochalasin B treatment. Transmission electron micrographs revealed no gross morphological changes at this dose. Absorptive junctional morphology was apparently not altered by cytochalasin B treatment, a finding which was consistent with the stable transepithelial electrical resistance observed during exposure to this drug. Active sodium transport processes coupled to hexose, amino acid, and chloride movements are all possible in L-15 medium. However, following exposure to 20 micrograms/ml cytochalasin B, all net sodium transport is completely inhibited. The data are consistent with the hypothesis of a common regulator for active sodium transport processes which is modulated through structural changes in cytoskeletal organization. |
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Authors:
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P T Beall; K J Karnaky; L T Garretson; Y J Kuo; L L Shanbour |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Biochimica et biophysica acta Volume: 763 ISSN: 0006-3002 ISO Abbreviation: Biochim. Biophys. Acta Publication Date: 1983 Aug |
Date Detail:
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Created Date: 1983-09-23 Completed Date: 1983-09-23 Revised Date: 2007-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0217513 Medline TA: Biochim Biophys Acta Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 19-26 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Biological Transport, Active / drug effects Cytochalasin B / pharmacology* Dimethyl Sulfoxide / pharmacology Epithelium / ultrastructure Intestinal Mucosa / drug effects, physiology*, ultrastructure Jejunum / drug effects, physiology* Male Membrane Potentials / drug effects Microscopy, Electron Rats Rats, Inbred Strains Sodium / metabolism* Sodium-Potassium-Exchanging ATPase / metabolism |
| Grant Support | |
ID/Acronym/Agency:
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AA00194-10/AA/NIAAA NIH HHS; GM29099/GM/NIGMS NIH HHS |
| Chemical | |
Reg. No./Substance:
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14930-96-2/Cytochalasin B; 67-68-5/Dimethyl Sulfoxide; 7440-23-5/Sodium; EC 3.6.3.9/Sodium-Potassium-Exchanging ATPase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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