Document Detail


Inhibition of the acrosome reaction (AR) and fertilization capacity of mouse spermatozoa by norethisterone A-ring reduced metabolite (5alpha-NET).
MedLine Citation:
PMID:  16164431     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The contra gestational effects of norethisterone and its main metabolites, 5alpha-NET and 3beta5alpha-NET, has been demonstrated in several species. However, the focus has been mainly on their effects in the uterus. We previously reported that 5alpha-NET inhibits the progesterone-induced AR in pig spermatozoa and induces severe morphological damage to fertilized mouse oocytes. In the present study, we analysed the effects of these compounds on the fertilization process in vitro. Oocytes and spermatozoa were obtained from Balb/c female and C57BL/6J male mice, respectively. Both, the AR assays and the fertilization experiments were performed under different steroid treatment schemes using progesterone as a control. Results showed that norethisterone induced the AR, while 5alpha-NET reduced the percentage of spermatozoa that had undergone progesterone-induced AR. Both 17beta-estradiol and 3beta5alpha-NET induced the AR in a considerably lower percentage of spermatozoa than progesterone. In addition, when 5alpha-NET was added to the medium simultaneously with progesterone at the moment of fertilization, the percentage of fertilized oocytes (two-cell stage) decreased by as much as 77% as compared with the control progesterone-treated group. All results suggest that these compounds can have important effects on the fertilization process.
Authors:
F-H Héctor; D C Paola; G Mora Gustavo; B G Arturo; C R Ivone
Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Andrologia     Volume:  37     ISSN:  0303-4569     ISO Abbreviation:  Andrologia     Publication Date:  2005 Aug 
Date Detail:
Created Date:  2005-09-16     Completed Date:  2005-12-23     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0423506     Medline TA:  Andrologia     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  135-42     Citation Subset:  IM    
Affiliation:
Departamento de Bioquímica y Biología Molecular, Instituto Nacional de Perinatología, Mexico City, Mexico.
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MeSH Terms
Descriptor/Qualifier:
Acrosome Reaction / drug effects*
Animals
Estradiol / pharmacology
Female
Fertilization / drug effects*
Male
Mice
Mice, Inbred BALB C
Norethindrone / analogs & derivatives,  pharmacology
Spermatozoa / drug effects*
Chemical
Reg. No./Substance:
50-28-2/Estradiol; 68-22-4/Norethindrone

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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