Document Detail


Inhibition of Tulane virus replication in vitro with RNA interference.
MedLine Citation:
PMID:  23154881     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
RNA interference (RNAi), a conserved mechanism triggered by small interfering RNA (siRNA), has been used for suppressing gene expression through RNA degradation. The replication of caliciviruses (CVs) with RNAi was studied using the Tulane virus (TV) as a model. Five siRNAs targeting the non-structural, the major (VP1) and minor (VP2) structural genes of the TV were developed and the viruses were quantified using quantitative real time PCR (qPCR) and tissue culture infective dose (TCID(50) ) assay. Treatment of the cells with siRNA 4 hr before viral inoculation significantly reduced viral titer by up to 2.6 logs and dramatically decreased viral RNA copy numbers and viral titers 48 hr post infection in four of the five siRNAs studied. The results were confirmed by Western blot, in which the major structural protein VP1 was markedly reduced in both the cells and the culture medium. Two small protein bands of the shell (S) and protruding (P) domains of the viral capsid protein were also detected in the cell lysates, although their role in viral replication remains unknown. Since the TV shares many biological properties with human noroviruses (NoVs), the successful demonstration of RNAi in TV replication would provide valuable information in control of acute gastroenteritis caused by human NoVs.
Authors:
Qiang Fan; Chao Wei; Ming Xia; Xi Jiang
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Journal of medical virology     Volume:  85     ISSN:  1096-9071     ISO Abbreviation:  J. Med. Virol.     Publication Date:  2013 Jan 
Date Detail:
Created Date:  2012-11-16     Completed Date:  2013-04-17     Revised Date:  2014-01-10    
Medline Journal Info:
Nlm Unique ID:  7705876     Medline TA:  J Med Virol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  179-86     Citation Subset:  IM    
Copyright Information:
Copyright © 2012 Wiley Periodicals, Inc.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Animals
Antiviral Agents / metabolism
Blotting, Western
Caliciviridae / genetics,  physiology*
Cell Line
Macaca mulatta
RNA Interference*
RNA, Small Interfering / genetics,  metabolism
RNA, Viral / genetics,  metabolism
Real-Time Polymerase Chain Reaction
Viral Load
Virus Replication*
Grant Support
ID/Acronym/Agency:
R01 AI037093/AI/NIAID NIH HHS; R01 AI055649/AI/NIAID NIH HHS; R01 AI055649/AI/NIAID NIH HHS; R01 AI089634/AI/NIAID NIH HHS; R01 AI089634/AI/NIAID NIH HHS; R01 AI37093/AI/NIAID NIH HHS
Chemical
Reg. No./Substance:
0/Antiviral Agents; 0/RNA, Small Interfering; 0/RNA, Viral
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Molecular characterization of serotype G9 rotaviruses circulating in South Korea between 2005 and 20...
Next Document:  Sorafenib inhibits in vitro osteoclastogenesis by down-modulating Mcl-1.