Document Detail


Inhibition of TPA-induced cyclooxygenase-2 expression and skin inflammation in mice by wogonin, a plant flavone from Scutellaria radix.
MedLine Citation:
PMID:  11502282     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Wogonin (5,7-dihydroxy-8-methoxyflavone), isolated from Scutellaria radix, was previously reported to inhibit the expression and activity of the enzyme cyclooxygenase-2 in lipopolysaccharide (LPS)-stimulated cells of a mouse macrophage cell line, RAW 264.7. Here, in order to find in vivo effects, inhibition by wogonin of 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced cyclooxygenase-2 expression and anti-inflammatory activity in vivo were investigated. When applied topically to the dorsal skin of mice, wogonin at doses of 50-200 microg/site/treatment (total of five treatments in 3 days) inhibited cyclooxygenase-2 expression and prostaglandin E2 production induced by multiple treatments with TPA. At 200 microg/site/treatment, wogonin caused a 55.3% reduction of prostaglandin E2 production on the dorsal skin compared with an increased production in the TPA-treated control group. The same compound significantly inhibited mouse ear edema induced by TPA in both preventive (58.1% inhibition) as well as curative treatment (31.3% inhibition) schedules at 200 microg/ear/treatment. Inhibition of neutrophil infiltration was also observed. Therefore, wogonin may be beneficial for cyclooxygenase-2-related skin disorders.
Authors:
B K Park; M Y Heo; H Park; H P Kim
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  European journal of pharmacology     Volume:  425     ISSN:  0014-2999     ISO Abbreviation:  Eur. J. Pharmacol.     Publication Date:  2001 Aug 
Date Detail:
Created Date:  2001-08-14     Completed Date:  2001-09-20     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  1254354     Medline TA:  Eur J Pharmacol     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  153-7     Citation Subset:  IM    
Affiliation:
College of Pharmacy, Kangwon National University, 200-701, Chunchon, South Korea.
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MeSH Terms
Descriptor/Qualifier:
Angiosperms / chemistry
Animals
Antioxidants / therapeutic use*
Cyclooxygenase 2
Dinoprostone / biosynthesis
Disease Models, Animal
Drugs, Chinese Herbal / therapeutic use
Edema / chemically induced,  metabolism,  prevention & control*
Flavanones*
Flavonoids / therapeutic use*
Gene Expression / drug effects
Isoenzymes / antagonists & inhibitors*,  biosynthesis
Male
Mice
Mice, Inbred ICR
Plant Extracts / chemistry
Prostaglandin-Endoperoxide Synthases / biosynthesis
Skin Diseases / chemically induced,  metabolism,  prevention & control*
Tetradecanoylphorbol Acetate / pharmacology
Chemical
Reg. No./Substance:
0/Antioxidants; 0/Drugs, Chinese Herbal; 0/Flavanones; 0/Flavonoids; 0/Isoenzymes; 0/Plant Extracts; 16561-29-8/Tetradecanoylphorbol Acetate; 363-24-6/Dinoprostone; 525-82-6/flavone; 632-85-9/wogonin; EC 1.14.99.1/Cyclooxygenase 2; EC 1.14.99.1/Prostaglandin-Endoperoxide Synthases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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