Document Detail

Inhibition of TNF-α by cyclophosphamide reduces myocardial injury after ischemia-reperfusion.
MedLine Citation:
PMID:  22971714     Owner:  NLM     Status:  MEDLINE    
PURPOSE: The purpose of this study was to determine whether cyclophosphamide (CP) can decrease myocardial and systemic TNF-α expression and thus protects myocardial I/R injury.
METHODS: Open chest rats were subjected to 30 min of ischemia followed by 3h, 12h or 24h of reperfusion. Rats were divided into sham group, I/R group and CP group, and each group included 3 timepoint subgroups (3h, 12h and 24h). Plasma TNF-α was measured by cytometric bead array (CBA) and immunohistochemistry was used to detect TNF-α in myocardium.
RESULTS: Compared with I/R group, rats treated with CP showed a significant difference with decreased plasma TNF-α (13.31 ± 2.62 vs 14.13 ± 5.95 pg/mL at 3 h reperfusion, 10.1 ± 2.73 vs 12.54 ± 5.00 pg/mL at 12 h reperfusion, 10.38 ± 5.59 vs 13.00 ± 3.59 pg/mL at 24 h reperfusion, p <0.05 respectively). Immunostaining was less intense with CP injection at each reperfusion time. The score of the intensity of myocardial TNF-α staining was down regulated.
CONCLUSIONS: TNF-α is expressed in the myocardium and plasma after myocardial I/R injury. CP might be a feasible strategy for anti-TNF-α to protect myocardial I/R injury.
Jianmei Jin; Fuxu Chen; Qiqi Wang; Yuangang Qiu; Lili Zhao; Zhidong Guo
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2012-08-20
Journal Detail:
Title:  Annals of thoracic and cardiovascular surgery : official journal of the Association of Thoracic and Cardiovascular Surgeons of Asia     Volume:  19     ISSN:  2186-1005     ISO Abbreviation:  Ann Thorac Cardiovasc Surg     Publication Date:  2013 Feb 
Date Detail:
Created Date:  2013-04-08     Completed Date:  2013-10-22     Revised Date:  2014-07-31    
Medline Journal Info:
Nlm Unique ID:  9703158     Medline TA:  Ann Thorac Cardiovasc Surg     Country:  Japan    
Other Details:
Languages:  eng     Pagination:  24-9     Citation Subset:  IM    
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MeSH Terms
Cyclophosphamide / pharmacology*
Disease Models, Animal
Flow Cytometry
Myocardial Infarction / blood,  immunology,  pathology,  prevention & control*
Myocardial Reperfusion Injury / blood,  immunology,  pathology,  prevention & control*
Myocardium / immunology*,  pathology
Time Factors
Tumor Necrosis Factor-alpha / antagonists & inhibitors*,  blood
Reg. No./Substance:
0/Tumor Necrosis Factor-alpha; 8N3DW7272P/Cyclophosphamide

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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