Document Detail

Inhibition of TNF-α by Cyclophosphamide Reduces Myocardial Injury After Ischemia-Reperfusion.
MedLine Citation:
PMID:  22971714     Owner:  NLM     Status:  Publisher    
Purpose: The purpose of this study was to determine whether cyclophosphamide (CP) can decrease myocardial and systemic TNF-α expression and thus protects myocardial I/R injury. Methods: Open chest rats were subjected to 30 min of ischemia followed by 3h, 12h or 24h of reperfusion. Rats were divided into sham group, I/R group and CP group, and each group included 3 timepoint subgroups (3h, 12h and 24h). Plasma TNF-αwas measured by cytometric bead array (CBA) and immunohistochemistry was used to detect TNF-α in myocardium. Results: Compared with I/R group, rats treated with CP showed a significant difference with decreased plasma TNF-α (13.31 ± 2.62 vs 14.13 ± 5.95 pg/mL at 3 h reperfusion, 10.1 ± 2.73 vs 12.54 ± 5.00 pg/mL at 12 h reperfusion, 10.38 ± 5.59 vs 13.00 ± 3.59 pg/mL at 24 h reperfusion, p <0.05 respectively). Immunostaining was less intense with CP injection at each reperfusion time. The score of the intensity of myocardial TNF-αstaining was down regulated.Conclusions: TNF-α is expressed in the myocardium and plasma after myocardial I/R injury. CP might be a feasible strategy for anti-TNF-α to protect myocardial I/R injury.
Jianmei Jin; Fuxu Chen; Qiqi Wang; Yuangang Qiu; Lili Zhao; Zhidong Guo
Related Documents :
20602474 - Preoperative coronary artery revascularization and long-term outcomes following abdomin...
8015324 - Impaired left ventricular function, one- or two-vessel coronary artery disease, and sev...
15227094 - Surgical intervention in acute myocardial infarction.
10793164 - Long-term treatment with a platelet glycoprotein-receptor antagonist after percutaneous...
8618604 - Prediction of the need for intensive care in patients who come to the emergency departm...
25073534 - Myocardial injury in a 41-year-old male treated with methylphenidate: a case report.
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-8-20
Journal Detail:
Title:  Annals of thoracic and cardiovascular surgery : official journal of the Association of Thoracic and Cardiovascular Surgeons of Asia     Volume:  -     ISSN:  2186-1005     ISO Abbreviation:  Ann Thorac Cardiovasc Surg     Publication Date:  2012 Aug 
Date Detail:
Created Date:  2012-9-13     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9703158     Medline TA:  Ann Thorac Cardiovasc Surg     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Department of Cardiology, the First Affiliated Hospital, College of Medicine, Zhejiang University, Institute of Cardiology, Zhejiang University, Hangzhou, People's Republic of China.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Effective Utilization of Chest X-ray for Follow-up of Metastatic Lung Tumor due to Soft Tissue Sarco...
Next Document:  Long-Term Results of Modified Bentall Procedure Using Flanged Composite Aortic Prosthesis.