| Inhibition of TNF-α by Cyclophosphamide Reduces Myocardial Injury After Ischemia-Reperfusion. | |
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MedLine Citation:
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PMID: 22971714 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Purpose: The purpose of this study was to determine whether cyclophosphamide (CP) can decrease myocardial and systemic TNF-α expression and thus protects myocardial I/R injury. Methods: Open chest rats were subjected to 30 min of ischemia followed by 3h, 12h or 24h of reperfusion. Rats were divided into sham group, I/R group and CP group, and each group included 3 timepoint subgroups (3h, 12h and 24h). Plasma TNF-αwas measured by cytometric bead array (CBA) and immunohistochemistry was used to detect TNF-α in myocardium. Results: Compared with I/R group, rats treated with CP showed a significant difference with decreased plasma TNF-α (13.31 ± 2.62 vs 14.13 ± 5.95 pg/mL at 3 h reperfusion, 10.1 ± 2.73 vs 12.54 ± 5.00 pg/mL at 12 h reperfusion, 10.38 ± 5.59 vs 13.00 ± 3.59 pg/mL at 24 h reperfusion, p <0.05 respectively). Immunostaining was less intense with CP injection at each reperfusion time. The score of the intensity of myocardial TNF-αstaining was down regulated.Conclusions: TNF-α is expressed in the myocardium and plasma after myocardial I/R injury. CP might be a feasible strategy for anti-TNF-α to protect myocardial I/R injury. |
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Authors:
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Jianmei Jin; Fuxu Chen; Qiqi Wang; Yuangang Qiu; Lili Zhao; Zhidong Guo |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2012-8-20 |
Journal Detail:
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Title: Annals of thoracic and cardiovascular surgery : official journal of the Association of Thoracic and Cardiovascular Surgeons of Asia Volume: - ISSN: 2186-1005 ISO Abbreviation: Ann Thorac Cardiovasc Surg Publication Date: 2012 Aug |
Date Detail:
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Created Date: 2012-9-13 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9703158 Medline TA: Ann Thorac Cardiovasc Surg Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
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Department of Cardiology, the First Affiliated Hospital, College of Medicine, Zhejiang University, Institute of Cardiology, Zhejiang University, Hangzhou, People's Republic of China. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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