Document Detail


Inhibition of Salmonella Enteritidis by cerein 8A, EDTA and sodium lactate.
MedLine Citation:
PMID:  19775768     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The ability of the bacteriocin cerein 8A to inhibit Salmonella Enteritidis in combination with EDTA and sodium lactate was investigated. Salmonella Enteritidis was incubated with combinations of cerein 8A (3200AU/mL) and EDTA (20, 50, 100 mmol/L) or sodium lactate (200 mmol/L). All treatments caused a significant reduction in the OD(600) values of Salmonella Enteritidis cultures. The addition of cerein 8A plus EDTA resulted in higher inhibition in comparison with the bacteriocin alone; the greater the concentration of EDTA, the greater the inhibitory effect. The combination of cerein 8A plus 100 mmol/L EDTA results in a more efficient treatment to reduce the number of viable cells of Salmonella Enteritidis. The combination of cerein 8A plus sodium lactate also showed significant inhibition of the indicator organism. Transmission electron microscopy showed damaged cell walls and loss of protoplasmic material in treated cells. The cells of Salmonella Enteritidis treated with cerein 8A plus EDTA appeared more injured. The bacteriocin cerein 8A may be useful to inhibit Gram-negative bacteria, with enhanced effect in combination with chelating agents. Control of Salmonella Enteritidis, a Gram-negative bacterium constantly linked to food outbreaks, addresses an important aspect of food safety.
Authors:
Rosiele Lappe; Amanda S Motta; Voltaire Sant'anna; Adriano Brandelli
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-09-12
Journal Detail:
Title:  International journal of food microbiology     Volume:  135     ISSN:  1879-3460     ISO Abbreviation:  Int. J. Food Microbiol.     Publication Date:  2009 Nov 
Date Detail:
Created Date:  2009-10-09     Completed Date:  2010-02-02     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8412849     Medline TA:  Int J Food Microbiol     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  312-6     Citation Subset:  IM    
Affiliation:
Laborat??rio de Bioqu??mica e Microbiologia Aplicada, Departamento de Ci??ncia de Alimentos, ICTA, Universidade Federal do Rio Grande do Sul, 91501-970 Porto Alegre, Brazil.
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MeSH Terms
Descriptor/Qualifier:
Anti-Infective Agents / pharmacology*
Bacteriocins / pharmacology*
Cell Wall / drug effects
Chelating Agents / pharmacology*
Colony Count, Microbial
Cytoplasm / drug effects
Dose-Response Relationship, Drug
Drug Therapy, Combination
Edetic Acid / pharmacology*
Microbial Viability / drug effects*
Microscopy, Electron, Transmission
Salmonella enteritidis / drug effects*,  growth & development
Sodium Lactate / pharmacology*
Chemical
Reg. No./Substance:
0/Anti-Infective Agents; 0/Bacteriocins; 0/Chelating Agents; 0/cerein 8A, Bacillus cereus; 60-00-4/Edetic Acid; 72-17-3/Sodium Lactate

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