Document Detail

Inhibition of Paracoccidioides brasiliensis by ajoene is associated with blockade of phosphatidylcholine biosynthesis.
MedLine Citation:
PMID:  9168609     Owner:  NLM     Status:  MEDLINE    
In Paracoccidioides brasiliensis, a dimorphic fungus pathogenic for humans, no significant differences were observed in the phospholipid species of both morphological phases. The species observed were phosphatidylcholine (PC, 30-40%), phosphatidylethanolamine (PE, 27-28%), phosphatidylserine (16-19%), phosphatidylinositol (13-17%) and sphingomyelin (3-5%). The main fatty acids found in the yeast (Y) phase were palmitate (56%), linoleate (18%) and oleate (15%), while linoleate predominated (61%) in the mycelial (M) phase, followed by palmitate (27%) and oleate (7%). In the Y phase the main free sterol was ergosta-5,22-dien-3 beta-ol (82%) plus some lanosterol (12%) and ergosterol (6%), while in the M phase, the latter predominated (88%), followed by low levels of ergosta-5,22-dien-3 beta-ol (12%). Ajoene [(E,Z)-4,5,9-trithiadodeca-1,6,11-triene 9-oxide], a platelet aggregation inhibitor derived from garlic, induced alterations in phospholipid and fatty acid proportions such that PC was reduced to about 18% in both phases and PE increased to 38% (Y phase) or 44% (M phase), suggesting inhibition of PC synthesis. Ajoene also reduced saturated fatty acids (16:0 and 18:0) from 67 to 35% in the Y phase, with a corresponding increase in the unsaturated components. This effect was not seen in the M phase.
G San-Blas; J A Urbina; E Marchán; L M Contreras; F Sorais; F San-Blas
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Microbiology (Reading, England)     Volume:  143 ( Pt 5)     ISSN:  1350-0872     ISO Abbreviation:  Microbiology (Reading, Engl.)     Publication Date:  1997 May 
Date Detail:
Created Date:  1997-07-21     Completed Date:  1997-07-21     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9430468     Medline TA:  Microbiology     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  1583-6     Citation Subset:  IM    
Instituto Venezolano de Investigaciones Cientificas (IVIC), Centres of Microbiology and Cell Biology, Caracas, Venezuela.
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MeSH Terms
Antifungal Agents / pharmacology*
Disulfides / pharmacology*
Fatty Acids / classification,  metabolism
Paracoccidioides / drug effects*,  growth & development,  metabolism,  ultrastructure
Phosphatidylcholines / biosynthesis*,  classification
Plant Extracts / pharmacology*
Reg. No./Substance:
0/Antifungal Agents; 0/Disulfides; 0/Fatty Acids; 0/Phosphatidylcholines; 0/Plant Extracts; 92285-01-3/ajoene
Erratum In:
Microbiology 1998 Jan;144 (Pt 1):261

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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