Document Detail


Inhibition of PTP1B by trodusquemine (MSI-1436) causes fat-specific weight loss in diet-induced obese mice.
MedLine Citation:
PMID:  20075852     Owner:  NLM     Status:  In-Process    
Abstract/OtherAbstract:
Trodusquemine (MSI-1436) causes rapid and reversible weight loss in genetic models of obesity. To better predict the potential effects of trodusquemine in the clinic, we investigated the effects of trodusquemine treatment in a murine model of diet-induced obesity (DIO). Trodusquemine suppressed appetite, reduced body weight (BW) in a fat-specific manner, and improved plasma insulin and leptin levels in mice. Screening assays revealed that trodusquemine selectively inhibited protein-tyrosine phosphatase 1B (PTP1B), a key enzyme regulating insulin and leptin signaling. Trodusquemine significantly enhanced insulin-stimulated tyrosine phosphorylation of insulin receptor (IR) beta and STAT3, direct targets of PTP1B, in HepG2 cells in vitro and/or hypothalamic tissue in vivo. These data establish trodusquemine as an effective central and peripheral PTP1B inhibitor with the potential to elicit noncachectic fat-specific weight loss and improve insulin and leptin levels.
Authors:
Kristen A Lantz; Susan G Emeigh Hart; Sonia L Planey; Mitchell F Roitman; Inez A Ruiz-White; Henry R Wolfe; Michael P McLane
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2010-01-14
Journal Detail:
Title:  Obesity (Silver Spring, Md.)     Volume:  18     ISSN:  1930-7381     ISO Abbreviation:  Obesity (Silver Spring)     Publication Date:  2010 Aug 
Date Detail:
Created Date:  2010-07-28     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101264860     Medline TA:  Obesity (Silver Spring)     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1516-23     Citation Subset:  IM    
Affiliation:
Department of Preclinical Research, Genaera Corporation, Plymouth Meeting, Pennsylvania, USA.
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MeSH Terms
Descriptor/Qualifier:
Grant Support
ID/Acronym/Agency:
1R43DK066953-01/DK/NIDDK NIH HHS

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