Document Detail

Inhibition of PDGF-induced c-jun and c-fos expression by a tyrosine protein kinase inhibitor.
MedLine Citation:
PMID:  1900355     Owner:  NLM     Status:  MEDLINE    
The expression of the proto-oncogenes c-fos, c-jun, jun B and jun D was monitored in quiescent C3H10T1/2 fibroblasts after stimulation with PDGF. The mRNA level of c-fos, c-jun and jun B, but not of jun D, was stimulated by PDGF. The inductions were abolished when genistein, a specific tyrosine protein kinase inhibitor, was added concomitantly with PDGF, a condition in which DNA synthesis is known to be inhibited. As already shown previously, treatment with PDGF and genistein for 4h followed by the replacement with fresh medium induces the progression of the cells through the G1 phase of their growth-division cycle, without phospholipase C activation. The removal of PDGF and genistein was accompanied by an important increase in c-fos, c-jun and jun B mRNA expression, which correlated with the entrance of cells into G1 phase. Thus, the proto-oncogene expressions induced by PDGF are also obtained in the absence of phospholipase C activation. This result also suggests that the mRNA levels of c-jun, jun B and to a lesser degree c-fos are positively regulated by tyrosine protein kinase activity, whereas jun D is negatively regulated.
J Zwiller; P Sassone-Corsi; K Kakazu; A L Boynton
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Oncogene     Volume:  6     ISSN:  0950-9232     ISO Abbreviation:  Oncogene     Publication Date:  1991 Feb 
Date Detail:
Created Date:  1991-04-09     Completed Date:  1991-04-09     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  8711562     Medline TA:  Oncogene     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  219-21     Citation Subset:  IM    
Centre de Neurochimie, CNRS, Strasbourg, France.
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MeSH Terms
DNA-Binding Proteins / genetics*
G1 Phase
Gene Expression*
Isoflavones / pharmacology
Platelet-Derived Growth Factor / pharmacology*
Protein-Tyrosine Kinases / antagonists & inhibitors*
Proto-Oncogene Proteins / genetics*
Proto-Oncogene Proteins c-fos
Proto-Oncogene Proteins c-jun
RNA, Messenger / analysis
Transcription Factors / genetics*
Grant Support
Reg. No./Substance:
0/DNA-Binding Proteins; 0/Isoflavones; 0/Platelet-Derived Growth Factor; 0/Proto-Oncogene Proteins; 0/Proto-Oncogene Proteins c-fos; 0/Proto-Oncogene Proteins c-jun; 0/RNA, Messenger; 0/Transcription Factors; 446-72-0/Genistein; EC Kinases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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