Document Detail

Inhibition of PAF-acether effects on isolated guinea pig hearts by zinc ions (Zn2+).
MedLine Citation:
PMID:  8546883     Owner:  NLM     Status:  MEDLINE    
PAF-acether is a phospholipid synthesized by most animal tissues and exerting a strong decrease on the heart's contractile force and coronary flow. PAF-acether (10(-9) and 10(-10)M) was administered to isolated guinea pig hearts perfused via the Langendorff apparatus with Chenoweth solution. Zinc (1.5 microM) is known to benefit heart function thus, Zn2+ (1.5, 7.5, and 30 microM) was added in the perfusing solution before or after PAF-acether administration. Contractile force, coronary flow, and heart rate were recorded by means of a Narco MK-IV Physiograph throughout all modes of perfusion. Calcium inhibitor (Verapamil 10(-10)M) and Pb+2 Co2+ (1.5 x 10(-6)M) were used subsequently in the perfusing solutions in order to elucidate some of the Zn and PAF interactions observed. All hearts were analyzed for their Zn and Ca content by means of an Atomic Absorption Spectrophotometry (AAS). Our data suggest that low concentrations of zinc (1.5 microM) can strongly inhibit PAF-induced decrease of contractile force and coronary flow. Zinc-inhibiting effects on PAF's negative inotropic action (myocytic level) is not exerted through Zn-Ca antagonism. Nevertheless, a Zn-Ca antagonism in the arteriolar level cannot be excluded. Zinc inhibits PAF selectively only if it is administered before PAF injection and this strongly suggests a receptor interaction between the metal and the phospholipid at the heart level.
A Evangelou; V Kalfakakou; J Benveniste; B Arnoux
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Publication Detail:
Type:  In Vitro; Journal Article    
Journal Detail:
Title:  Biological trace element research     Volume:  50     ISSN:  0163-4984     ISO Abbreviation:  Biol Trace Elem Res     Publication Date:  1995 Oct 
Date Detail:
Created Date:  1996-02-21     Completed Date:  1996-02-21     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7911509     Medline TA:  Biol Trace Elem Res     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  43-55     Citation Subset:  IM    
Laboratory of Experimental Physiology, Faculty of Medicine, University of Ioannina, Greece.
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MeSH Terms
Calcium / metabolism
Cobalt / pharmacology
Coronary Circulation / drug effects
Guinea Pigs
Heart / drug effects*
Heart Rate / drug effects
Myocardial Contraction / drug effects
Myocardium / metabolism
Platelet Activating Factor / antagonists & inhibitors*,  pharmacology
Spectrophotometry, Atomic
Zinc / metabolism,  pharmacology*
Reg. No./Substance:
0/Platelet Activating Factor; 7440-48-4/Cobalt; 7440-66-6/Zinc; 7440-70-2/Calcium

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