Document Detail


Inhibition of NADPH oxidase attenuates vasospasm after experimental subarachnoid hemorrhage in rats.
MedLine Citation:
PMID:  15817890     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND AND PURPOSE: The purpose of this study is to investigate whether apocynin, an NADPH oxidase inhibitor, attenuates vasospasm after experimental subarachnoid hemorrhage (SAH) in rats.
METHODS: Rats were subjected to endovascular perforation of the right anterior cerebral artery or sham surgery. Beginning 2 hours after SAH, rats were administered 50 mg/kg apocynin or vehicle by intraperitoneal injection 3 times daily for 2 days.
RESULTS: In SAH rats, apocynin treatment enlarged basilar artery diameter (SAH/apocynin=253+/-71 microm, SAH/saline=191+/-60 microm, P<0.01; SAH=190+/-58 microm, sham=276+/-52 microm, P<0.01), reduced neurological deficits (SAH/apocynin=24+/-6.5, SAH/saline=18+/-5.3, P<0.05; SAH=18+/-4.7, sham=27+/-0, P<0.01), decreased NADPH oxidase activity (SAH/apocynin=18.4+/-3.7, SAH/saline=25.7+/-5.2, P<0.05; SAH=27.5+/-5.8, sham=15.4+/-4.5 nmol/min per mg protein, P<0.05), decreased superoxide level (SAH/apocynin=6.5+/-1.8, SAH/saline=9.6+/-2.2, P<0.05; SAH=9.8+/-1.9, sham=4.9+/-0.9 arbitrary units, P<0.05), and lowered membrane translocation of NADPH oxidase subunit p47phox.
CONCLUSIONS: Inhibition of NADPH oxidase attenuates delayed cerebral vasospasm after experimental SAH, suggesting that the inhibition of NADPH oxidase may provide a therapeutic strategy for vasospasm after SAH.
Authors:
Jie-Sheng Zheng; Ren-Ya Zhan; Shu-Sen Zheng; Yong-Qing Zhou; Ying Tong; Shu Wan
Publication Detail:
Type:  Journal Article; Retracted Publication    
Journal Detail:
Title:  Stroke; a journal of cerebral circulation     Volume:  36     ISSN:  1524-4628     ISO Abbreviation:  Stroke     Publication Date:  2005 May 
Date Detail:
Created Date:  2005-05-04     Completed Date:  2005-06-23     Revised Date:  2011-08-18    
Medline Journal Info:
Nlm Unique ID:  0235266     Medline TA:  Stroke     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1059-64     Citation Subset:  IM    
Affiliation:
Department of Neurosurgery, The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, China. zhengjiesheng@126.com
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MeSH Terms
Descriptor/Qualifier:
Acetophenones / therapeutic use*
Animals
Basilar Artery / drug effects,  pathology
Enzyme Inhibitors / therapeutic use*
Male
NADPH Oxidase / antagonists & inhibitors*
Phosphoproteins / metabolism
Protein Transport / drug effects
Rats
Rats, Sprague-Dawley
Subarachnoid Hemorrhage / complications*
Superoxides / metabolism
Vasospasm, Intracranial / drug therapy*,  etiology,  metabolism
Chemical
Reg. No./Substance:
0/Acetophenones; 0/Enzyme Inhibitors; 0/Phosphoproteins; 11062-77-4/Superoxides; 498-02-2/acetovanillone; EC 1.6.3.1/NADPH Oxidase; EC 1.6.3.1/neutrophil cytosolic factor 1
Comments/Corrections
Retraction In:
Stroke. 2011 Jul;42(7):2117   [PMID:  21709285 ]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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