Document Detail

Inhibition of MAP2 expression affects both morphological and cell division phenotypes of neuronal differentiation.
MedLine Citation:
PMID:  1997209     Owner:  NLM     Status:  MEDLINE    
Expression of the differentiated neuronal phenotype is typically manifest in several properties: distinct morphologies and organizations of the underlying cytoskeleton; appearance of specific macromolecules; and cessation of cell division. All of these properties are induced in undifferentiated embryonal carcinoma cells exposed to retinoic acid. We show here that the mRNA and protein for the microtubule component MAP2 is also induced by retinoic acid. Stable transfectants of undifferentiated cells, constitutively expressing MAP2 antisense RNA, show significantly reduced levels of MAP2 antisense RNA, show significantly reduced levels of MAP2 protein upon induction compared with controls. These cells do express other neuronal markers, but they do not undergo normal morphological differentiation nor do they withdraw from the cell cycle. The results suggest that MAP2 expression may be necessary for both neurite extension and cessation of cell division.
J H Dinsmore; F Solomon
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Cell     Volume:  64     ISSN:  0092-8674     ISO Abbreviation:  Cell     Publication Date:  1991 Feb 
Date Detail:
Created Date:  1991-04-02     Completed Date:  1991-04-02     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0413066     Medline TA:  Cell     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  817-26     Citation Subset:  IM    
Center for Cancer Research, Massachusetts Institute of Technology, Cambridge 02139.
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MeSH Terms
Antibodies, Monoclonal / diagnostic use
Cell Differentiation
Cell Division
Cell Line
Fluorescent Antibody Technique
Gene Expression
Microtubule-Associated Proteins / analysis,  genetics*
Neurons / cytology,  physiology*
RNA, Antisense / genetics
Restriction Mapping
Reg. No./Substance:
0/Antibodies, Monoclonal; 0/Microtubule-Associated Proteins; 0/RNA, Antisense

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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