Document Detail

Inhibition of L-type amino acid transporter modulates the expression of cell cycle regulatory factors in KB oral cancer cells.
MedLine Citation:
PMID:  20606299     Owner:  NLM     Status:  MEDLINE    
The purpose of this study was to examine the effect of 2-aminobicyclo-(2,2,1)-heptane-2-carboxylic acid (BCH), an inhibitor of L-type amino acid transporters, on the cell growth suppression in KB human oral cancer cells and to study the roles of cell cycle regulatory factors in the BCH-induced growth inhibition. The effect of BCH on cell growth suppression and the influence of BCH to cell cycle regulatory factors in KB cell growth inhibition were examined using cell cycle analysis, immunoblotting and immunoprecipitation. The BCH treatment induced cell cycle arrest at G1 phase in KB cells. The expression of cyclin D3 was remarkably decreased by BCH treatment. The BCH inhibited the expression of cyclin-dependent protein kinase 6 (CDK6) in a time-dependent manner. In addition, the expression of CDK inhibitor p27 was increased by BCH treatment in KB cells, but not CDK inhibitors p21 and p15. These results suggest that, in KB cells, the inhibition of LAT1 by BCH causes cell cycle arrest at G1 phase by inhibiting cyclin D3-CDK6 complex whereas increasing expression of a CDK inhibitor p27.
Chun Sung Kim; In-Sung Moon; Ju-Hyun Park; Woo-Cheol Shin; Hong Sung Chun; Sook-Young Lee; Joong-Ki Kook; Heung-Joong Kim; Joo-Cheol Park; Hitoshi Endou; Yoshikatsu Kanai; Byung-Kwon Lee; Do Kyung Kim
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biological & pharmaceutical bulletin     Volume:  33     ISSN:  1347-5215     ISO Abbreviation:  Biol. Pharm. Bull.     Publication Date:  2010  
Date Detail:
Created Date:  2010-07-07     Completed Date:  2010-11-26     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9311984     Medline TA:  Biol Pharm Bull     Country:  Japan    
Other Details:
Languages:  eng     Pagination:  1117-21     Citation Subset:  IM    
Department of Oralbiochemistry, Chosun University School of Dentistry, Dong-gu, Gwangju 501-759, Korea.
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MeSH Terms
Amino Acid Transport Systems / antagonists & inhibitors*
Blotting, Western
Cell Cycle Proteins / physiology*
Cell Line, Tumor
Mouth Neoplasms / pathology*
Reg. No./Substance:
0/Amino Acid Transport Systems; 0/Cell Cycle Proteins

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