| Inhibition of histone deacetylase activity in reduced oxygen environment enhances the osteogenesis of mouse adipose-derived stromal cells. | |
| | |
MedLine Citation:
|
PMID: 19505250 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Recent studies suggest that oxygen tension has a great impact on the osteogenic differentiation capacity of mesenchymal cells derived from adipose tissue: reduced oxygen impedes osteogenesis. We have found that expansion of mouse adipose-derived stromal cells (mASCs) in reduced oxygen tension (10%) results in increased cell proliferation along with induction of histone deacetylase (HDAC) activity. In this study, we utilized two HDAC inhibitors (HDACi), sodium butyrate (NaB) and valproic acid (VPA), and studied their effects on mASCs expanded in various oxygen tensions (21%, 10%, and 1% O(2)). Significant growth inhibition was observed with NaB or VPA treatment in each oxygen tension. Osteogenesis was enhanced by treatment with NaB or VPA, particularly in reduced oxygen tensions (10% and 1% O(2)). Conversely, adipogenesis was decreased with treatments of NaB or VPA at all oxygen tensions. Finally, NaB- or VPA-treated, reduced oxygen tension-exposed (1% O(2)) ASCs were grafted into surgically created mouse tibial defects and resulted in significantly increased bone regeneration. In conclusion, HDACi significantly promote the osteogenic differentiation of mASCs exposed to reduced oxygen tension; HDACi may hold promise for future clinical applications of ASCs for skeletal regeneration. |
| | |
Authors:
|
Yue Xu; Kyle E Hammerick; Aaron W James; Antoine L Carre; Philipp Leucht; Amato J Giaccia; Michael T Longaker |
Publication Detail:
|
Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: Tissue engineering. Part A Volume: 15 ISSN: 1937-335X ISO Abbreviation: Tissue Eng Part A Publication Date: 2009 Dec |
Date Detail:
|
Created Date: 2009-12-09 Completed Date: 2010-02-03 Revised Date: 2011-03-03 |
Medline Journal Info:
|
Nlm Unique ID: 101466659 Medline TA: Tissue Eng Part A Country: United States |
Other Details:
|
Languages: eng Pagination: 3697-707 Citation Subset: IM |
Affiliation:
|
Plastic and Reconstructive Surgery Division, Hagey Pediatric Regenerative Research Laboratory, Department of Surgery, Stanford University School of Medicine, Stanford University, Stanford, California 94305-5148, USA. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Adipogenesis
/
drug effects Adipose Tissue / cytology* Alkaline Phosphatase / metabolism Animals Bromodeoxyuridine / metabolism Butyrates / pharmacology Cell Proliferation / drug effects Gene Expression Regulation / drug effects Histone Deacetylase Inhibitors / pharmacology* Histone Deacetylases / metabolism* Mice Osteogenesis / drug effects*, genetics Oxygen / analysis* Stromal Cells / cytology*, drug effects, enzymology*, transplantation Tibia / drug effects, pathology Valproic Acid / pharmacology |
| Grant Support | |
ID/Acronym/Agency:
|
R01 DE-13194/DE/NIDCR NIH HHS; R01 DE-14526/DE/NIDCR NIH HHS |
| Chemical | |
Reg. No./Substance:
|
0/Butyrates; 0/Histone Deacetylase Inhibitors; 59-14-3/Bromodeoxyuridine; 7782-44-7/Oxygen; 99-66-1/Valproic Acid; EC 3.1.3.1/Alkaline Phosphatase; EC 3.5.1.98/Histone Deacetylases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: A never-ending love story with elastin: a scientific autobiography.
Next Document: A hollow sphere soft lithography approach for long-term hanging drop methods.