| Inhibition of histidine ammonia lyase by 8-methoxypsoralen and psoralen-oxidized photoproducts. | |
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MedLine Citation:
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PMID: 20880231 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The effect of 8-methoxypsoralen-UVA therapy on the catalysis of histidine to trans-urocanic acid by histidine ammonia lyase (HAL, EC 4.3.1.3) was examined using an enzymatic assay from Sigma-Aldrich where the growth of the trans-urocanic acid peak at 277 nm was monitored. A Rayonet Photochemical Mini Reactor (Model RMR-600) equipped with eight, 3500 Å light sources and a custom UVA filter (Model S-BAL3 2.9 mm), from the Solar Light Company, were used to expose various reaction mixtures to broadband UVA light and UVA/UVB light. A UV-Vis spectrophotometer (Model Shimadzu UV 2540) with a temperature-controlled cell holder (Model TCC240) was used to monitor the growth of the trans-urocanic peak. Results of dark-binding experiments of 8-methoxypsoralen in denatured ethanol indicate no inhibition of enzyme activity due to ethanol but noncompetitive inhibition due to 8-methoxypsoralen. The effects of preirradiated 8-methoxypsoralen, with both broadband UVA and UVA/UVB, indicate that inhibition was due to psoralen-oxidized photoproducts. Inhibition of HAL was found when exposed to broadband UVA/UVB and to a lesser extent when exposed to broadband UVA. |
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Authors:
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John T Reilly; Kyle A Troester; Trista T Tyner; Dominick A Vitale; Tiffiany R Risher |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S. Date: 2010-09-29 |
Journal Detail:
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Title: Photochemistry and photobiology Volume: 86 ISSN: 1751-1097 ISO Abbreviation: Photochem. Photobiol. Publication Date: 2010 Nov-Dec |
Date Detail:
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Created Date: 2010-11-24 Completed Date: 2011-03-21 Revised Date: 2011-04-11 |
Medline Journal Info:
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Nlm Unique ID: 0376425 Medline TA: Photochem Photobiol Country: United States |
Other Details:
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Languages: eng Pagination: 1272-7 Citation Subset: IM |
Copyright Information:
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© 2010 The Authors. Journal Compilation. The American Society of Photobiology. |
Affiliation:
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Department of Chemistry and Physics, Coastal Carolina University, Conway, SC, USA. jreilly@coastal.edu |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Histidine Ammonia-Lyase
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antagonists & inhibitors* Humans Immunosuppression Methoxsalen / pharmacology* Oxidation-Reduction PUVA Therapy Photochemical Processes Photosensitizing Agents / pharmacology Skin Diseases / drug therapy, enzymology, immunology Urocanic Acid / metabolism |
| Chemical | |
Reg. No./Substance:
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0/Photosensitizing Agents; 104-98-3/Urocanic Acid; 298-81-7/Methoxsalen; EC 4.3.1.3/Histidine Ammonia-Lyase |
| Comments/Corrections | |
Erratum In:
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Photochem Photobiol. 2011 Mar-Apr;87(2):487 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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