Document Detail


Inhibition of Gal beta1, 4GlcNAc alpha2,6 sialyltransferase expression by antisense-oligodeoxynucleotides.
MedLine Citation:
PMID:  9224687     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Treatment of human colorectal carcinoma cells HT29 with specific antisense oligodeoxynucleotides led to a decreased Gal beta1,4GlcNAc alpha2,6 sialyltransferase activity on the level of protein expression as well as on the mRNA level. Antisense treatment did not effect cell viability or cell growth. Oligodeoxynucleotides which were complementary to the region upstream of the initiation codon were particularly effective in inhibition of enzyme expression. No such inhibition was found by treatment of cells with oligodeoxynucleotides complementary to the region downstream of the initiation codon or by treatment of cells with scrambled controls or sense oligodeoxynucleotides.
Authors:
W Kemmner; K Hohaus; P M Schlag
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  FEBS letters     Volume:  409     ISSN:  0014-5793     ISO Abbreviation:  FEBS Lett.     Publication Date:  1997 Jun 
Date Detail:
Created Date:  1997-08-01     Completed Date:  1997-08-01     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0155157     Medline TA:  FEBS Lett     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  347-50     Citation Subset:  IM    
Affiliation:
Department of Surgical Oncology, Robert-Rössle-Klinik at the Max-Delbrück-Centrum for Molecular Medicine, Berlin, Germany. kemmnerw@rrkchir.mdc-berlin.de
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MeSH Terms
Descriptor/Qualifier:
Cell Division / drug effects
Enzyme Activation / drug effects,  genetics
HT29 Cells
Humans
Oligodeoxyribonucleotides / pharmacology*
Oligonucleotides, Antisense / pharmacology*
RNA, Messenger / antagonists & inhibitors,  drug effects
Sialyltransferases / antagonists & inhibitors*,  biosynthesis*,  genetics
Chemical
Reg. No./Substance:
0/Oligodeoxyribonucleotides; 0/Oligonucleotides, Antisense; 0/RNA, Messenger; EC 2.4.99.-/Sialyltransferases; EC 2.4.99.1/beta-D-galactoside alpha 2-6-sialyltransferase

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