Document Detail

Inhibition of Dictyostelium discoideum differentiation in monolayers in vitro by endogenous and exogenous lectins.
MedLine Citation:
PMID:  6688079     Owner:  NLM     Status:  MEDLINE    
Spore-cell differentiation in monolayers in vitro of two sporagenous mutants of Dictyostelium discoideum, HM18 and HM15, is markedly inhibited by relatively low concentrations of the exogenous lectins, Concanavalin A (ConA) and wheat germ agglutinin (WGA) and by somewhat higher concentrations of the endogenous lectin, discoidin. The selective inhibition of spore cell formation by ConA occurs toward the end of the differentiation process, as shown by studies involving the addition or removal of ConA at various times during the period of differentiation. In addition, ConA has no deleterious effect on the expression of the pre-spore cell specific enzyme, UDP-galactosyl transferase. It is likely, therefore, that the maturation of pre-spore cells into spore cells is the most sensitive locus of lectin inhibition. At higher concentrations of WGA and ConA, both stalk- and spore-cell formation are inhibited, but increased concentrations of discoidin do not have this effect.
K Laroy; G Weeks
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of cell science     Volume:  59     ISSN:  0021-9533     ISO Abbreviation:  J. Cell. Sci.     Publication Date:  1983 Jan 
Date Detail:
Created Date:  1983-08-11     Completed Date:  1983-08-11     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0052457     Medline TA:  J Cell Sci     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  203-12     Citation Subset:  IM    
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MeSH Terms
Carrier Proteins / pharmacology
Concanavalin A / pharmacology
Dictyostelium / cytology*,  drug effects
Lectins / pharmacology*
Protozoan Proteins*
Spores, Fungal / drug effects
Wheat Germ Agglutinins
Reg. No./Substance:
0/Carrier Proteins; 0/Lectins; 0/Protozoan Proteins; 0/Wheat Germ Agglutinins; 0/discoidins; 11028-71-0/Concanavalin A

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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