| Inhibition of DNA methyltransferase by microbial inhibitors and fatty acids. | |
| | |
MedLine Citation:
|
PMID: 8872747 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Streptomyces sp. strain No. 560 produces four kinds of DNA methyltransferase inhibitors in the culture filtrate. One of them, DMI-4 was distinguished from DMI-1, -2 and -3 previously reported with respect to certain properties, DMI-4 is considered to be a triglyceride consisting of the fatty acids anteisopentadecanoic acid (C15:0), isopalmitic acid (C16:0) and isostearic acid (C18:0) from the results of gas chromatography analysis. Since DMI-4 contains three molecules of fatty acid, and the previously reported DMI-1, 8-methylpentadecanoic acid, is analogous to a fatty acid, the inhibitory activity has been examined of various fatty acids and their methyl esters against Eco RI DNA methyltransferase (M. Eco RI). Oleic acid (C18:1) was found to be a potent inhibiton of M. Eco RI. The inhibitory activity of oleic acid was shown to be pH- and temperature-dependent and inhibited M. Eco RI in a noncompetitive manner with respect to DNA or S-adenosylmethionine (SAM). The number of carbon atoms and double bonds in the fatty acid molecule affected the inhibitory activity, but their methyl esters were not inhibitors. Our results suggest that the length of the carbon chain, the number of double bonds and the presence of a carboxyl group and branched methyl group in the fatty acid molecule may play an important role in the inhibition of DNA methyltransferase. |
| | |
Authors:
|
K Suzuki; K Nagao; J Tokunaga; N Katayama; M Uyeda |
Related Documents
:
|
10826817 - Staleya guttiformis gen. nov., sp. nov. and sulfitobacter brevis sp. nov., alpha-3-prot... 17503367 - The repertoire of desaturases for unsaturated fatty acid synthesis in 397 genomes. 10872837 - A palmitoyl-coa-specific delta9 fatty acid desaturase from caenorhabditis elegans. 12005057 - Identification and expression of a rat fatty acid elongase involved in the biosynthesis... 17097337 - Resonance light scattering technique for the determination of proteins with polymethacr... 5636347 - Autocorrelation functions of noisy electron micrographs of stained polynucleotide chains. |
Publication Detail:
|
Type: Journal Article |
Journal Detail:
|
Title: Journal of enzyme inhibition Volume: 10 ISSN: 8755-5093 ISO Abbreviation: J. Enzym. Inhib. Publication Date: 1996 |
Date Detail:
|
Created Date: 1997-01-02 Completed Date: 1997-01-02 Revised Date: 2001-11-28 |
Medline Journal Info:
|
Nlm Unique ID: 8709734 Medline TA: J Enzyme Inhib Country: SWITZERLAND |
Other Details:
|
Languages: eng Pagination: 271-280 Citation Subset: IM |
Affiliation:
|
Laboratory Medicinal Microbiology, Faculty of Pharmaceutical Sciences, Kumamoto University, Japan. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
DNA Modification Methylases
/
antagonists & inhibitors* Enzyme Inhibitors / chemistry, isolation & purification, pharmacology* Escherichia coli / enzymology Esters / pharmacology Fatty Acids / pharmacology* Hydrogen-Ion Concentration Kinetics Magnetic Resonance Spectroscopy Streptomyces / chemistry* Temperature Triglycerides / chemistry, pharmacology |
| Chemical | |
Reg. No./Substance:
|
0/8-methylpentadecanoic acid; 0/Enzyme Inhibitors; 0/Esters; 0/Fatty Acids; 0/Triglycerides; EC 2.1.1.-/DNA Modification Methylases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Inhibition of camel lens zeta-crystallin/NADPH:quinone oxidoreductase activity by Cibacron blue.
Next Document: Interaction of tissue plasminogen activator inhibitor with cell surface guanidinobenzoatase and urok...