Document Detail


Inhibition of DNA, RNA and protein synthesis and chromatin alteration by N-hydroxyphenacetin.
MedLine Citation:
PMID:  2435067     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The effects of N-hydroxyphenacetin on DNA function and structure were investigated to elucidate the involvement of phenacetin in analgesic nephropathy and transitional cell carcinoma. N-Hydroxyphenacetin or a metabolite inhibited synthesis of DNA, RNA and protein; DNA inhibition was greater at higher pH. No single-strand breaks were detectable in DNA after N-hydroxyphenacetin treatment and no appreciable effect on cell viability was observed at concentrations up to 5 mM. N-Hydroxyphenacetin-induced alteration to chromatin structure was detected using nucleoid sedimentation analysis. Direct binding to plasmid DNA was not observed. These observations are consistent with a role for phenacetin metabolites in renal disease.
Authors:
N K Hayward; M F Lavin
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Xenobiotica; the fate of foreign compounds in biological systems     Volume:  17     ISSN:  0049-8254     ISO Abbreviation:  Xenobiotica     Publication Date:  1987 Jan 
Date Detail:
Created Date:  1987-04-10     Completed Date:  1987-04-10     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  1306665     Medline TA:  Xenobiotica     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  115-24     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Cell Line
Cell Survival / drug effects
Chromatin / drug effects*
DNA / biosynthesis
DNA Damage
DNA Repair / drug effects
Humans
Kidney Diseases / chemically induced
Phenacetin / analogs & derivatives*,  pharmacology
Protein Biosynthesis
RNA / biosynthesis
Chemical
Reg. No./Substance:
0/Chromatin; 19315-64-1/N-hydroxyphenacetin; 62-44-2/Phenacetin; 63231-63-0/RNA; 9007-49-2/DNA

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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