| Inhibition of a C-rich oligodeoxynucleotide on activation of immune cells in vitro and enhancement of antibody response in mice. | |
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MedLine Citation:
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PMID: 20646074 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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To explore the possibility that human mitochondrial genomic DNA-mimicking oligodeoxynucleotides could regulate the immune response, a series of mitochondrial DNA-based oligodeoxynucleotides (MTODNs) were designed and studied to determine their immunoregulatory effects on immune cells activated by toll-like receptor (TLR) stimulation. The results showed that a C-rich MTODN, designated MT01, was able to inhibit the proliferation of human peripheral blood mononuclear cells (PBMCs) induced by cytosine-phosphate-guanosine (CpG) oligodeoxynucleotides (ODNs) and the production of type I interferon (IFN) from human PBMCs stimulated by TLR agonists, including inactivated influenza virus, imiquimod, inactivated herpes simplex virus-1 (HSV-1) and CpG ODNs. In addition, MT01 inhibited the CpG ODN-enhanced antibody response and this inhibition could be related to the antagonism of TLR9-activation pathways in B cells. Notably, unlike the G-rich suppressive ODNs reported, MT01 is composed of ACCCCCTCT repeats. These data imply that MT01 represents a novel class of immunosuppressive ODNs that could be candidate biologicals with therapeutic use in TLR activation-associated diseases. |
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Authors:
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Guang Yang; Min Wan; Yongsheng Zhang; Luguo Sun; Ran Sun; Dali Hu; Xiaojing Zhou; Li Wang; Xiuli Wu; Liying Wang; Yongli Yu |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Immunology Volume: 131 ISSN: 1365-2567 ISO Abbreviation: Immunology Publication Date: 2010 Dec |
Date Detail:
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Created Date: 2010-11-08 Completed Date: 2010-12-10 Revised Date: 2011-12-21 |
Medline Journal Info:
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Nlm Unique ID: 0374672 Medline TA: Immunology Country: England |
Other Details:
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Languages: eng Pagination: 501-12 Citation Subset: IM |
Copyright Information:
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© 2010 The Authors. Immunology © 2010 Blackwell Publishing Ltd. |
Affiliation:
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Department of Molecular Biology, Medical College of Norman Bethune, Jilin University, Changchun, China. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adjuvants, Immunologic
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pharmacology Aminoquinolines / pharmacology Animals Antibody Formation / drug effects*, immunology B-Lymphocytes / immunology* Cell Proliferation / drug effects* Cercopithecus aethiops DNA, Mitochondrial / pharmacology* Female Herpesvirus 1, Human / immunology Humans Immunosuppressive Agents / pharmacology* Interferon Type I / immunology Lymphocyte Activation / drug effects*, immunology Mice Mice, Inbred BALB C Oligodeoxyribonucleotides / pharmacology* Orthomyxoviridae / immunology Toll-Like Receptor 9 / agonists, immunology Vero Cells |
| Chemical | |
Reg. No./Substance:
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0/Adjuvants, Immunologic; 0/Aminoquinolines; 0/CPG-oligonucleotide; 0/DNA, Mitochondrial; 0/Immunosuppressive Agents; 0/Interferon Type I; 0/Oligodeoxyribonucleotides; 0/TLR9 protein, human; 0/Tlr9 protein, mouse; 0/Toll-Like Receptor 9; 99011-02-6/imiquimod |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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