Document Detail


Inhibition of biological activity of staphylococcal enterotoxin A (SEA) by apple juice and apple polyphenols.
MedLine Citation:
PMID:  20402509     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The foodborne pathogen Staphylococcus aureus produces the virulent staphylococcal enterotoxin A (SEA), a single-chain protein that consists of 233 amino acid residues with a molecular weight of 27 078 Da. SEA is a superantigen that is reported to contribute to animal (mastitis) and human (emesis, diarrhea, atopic dermatitis, arthritis, and toxic shock) syndromes. Changes of the native structural integrity may inactivate the toxin by preventing molecular interaction with cell membrane receptor sites of their host cells. In the present study, we evaluated the ability of one commercial and two freshly prepared apple juices and a commercial apple polyphenol preparation (Apple Poly) to inhibit the biological activity of SEA. Dilutions of freshly prepared apple juices and Apple Poly inhibited the biological activity of SEA without any significant cytotoxic effect on the spleen cells. Additional studies with antibody-coated immunomagnetic beads bearing specific antibodies against the toxin revealed that SEA added to apple juice appears to be largely irreversibly bound to the juice constituents. The results suggest that food-compatible and safe anti-toxin phenolic compounds can be used to inactivate SEA in vitro and possibly also in vivo, even after induction of T-cell proliferation by long-term exposure to SEA. The significance of the results for microbial food safety and human health is discussed.
Authors:
Reuven Rasooly; Paula M Do; Mendel Friedman
Related Documents :
12631899 - Surveillance of foodborne botulism in poland: 1960-1998.
8396079 - Revival of bacillus subtilis spores from biocide-induced injury in the germination proc...
20961439 - Universal and specific quantitative detection of botulinum neurotoxin genes.
12752519 - A double-blinded, randomized, placebo-controlled pilot study of the safety and efficacy...
14501999 - Food poisoning associated with biotoxins in fish and shellfish.
1957979 - Endemic food-borne botulism: clinical experience, 1973-1986 at alaska native medical ce...
24839969 - One solution for two challenges: the lizard microlophus atacamensis avoids overheating ...
17138079 - Implication of the anatomy of the pericardial reflection on positioning of central veno...
3221839 - Diversity of non-histone protein fraction nhcp2 from hamster kirkman-robbins hepatoma a...
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of agricultural and food chemistry     Volume:  58     ISSN:  1520-5118     ISO Abbreviation:  J. Agric. Food Chem.     Publication Date:  2010 May 
Date Detail:
Created Date:  2010-05-05     Completed Date:  2010-08-06     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0374755     Medline TA:  J Agric Food Chem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  5421-6     Citation Subset:  IM    
Affiliation:
Western Regional Research Center, Agricultural Research Service, United States Department of Agriculture, Albany, California 94710, USA. reuven.rasooly@ars.usda.gov
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Animals
Beverages*
Cell Division / drug effects
Enterotoxins / antagonists & inhibitors*
Female
Flavonoids / pharmacology*
Malus / chemistry*
Mice
Mice, Inbred C57BL
Phenols / pharmacology*
Spleen / drug effects
Chemical
Reg. No./Substance:
0/Enterotoxins; 0/Flavonoids; 0/Phenols; 0/polyphenols; 37337-57-8/enterotoxin A, Staphylococcal

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Soybean Tocopherol Concentrations Are Affected by Crop Management.
Next Document:  Immobilization of Heavy Metal Ions (Cu(II), Cd(II), Ni(II), and Pb(II)) by Broiler Litter-Derived Bi...