Document Detail

Inhibition of biological activity of staphylococcal enterotoxin A (SEA) by apple juice and apple polyphenols.
MedLine Citation:
PMID:  20402509     Owner:  NLM     Status:  MEDLINE    
The foodborne pathogen Staphylococcus aureus produces the virulent staphylococcal enterotoxin A (SEA), a single-chain protein that consists of 233 amino acid residues with a molecular weight of 27 078 Da. SEA is a superantigen that is reported to contribute to animal (mastitis) and human (emesis, diarrhea, atopic dermatitis, arthritis, and toxic shock) syndromes. Changes of the native structural integrity may inactivate the toxin by preventing molecular interaction with cell membrane receptor sites of their host cells. In the present study, we evaluated the ability of one commercial and two freshly prepared apple juices and a commercial apple polyphenol preparation (Apple Poly) to inhibit the biological activity of SEA. Dilutions of freshly prepared apple juices and Apple Poly inhibited the biological activity of SEA without any significant cytotoxic effect on the spleen cells. Additional studies with antibody-coated immunomagnetic beads bearing specific antibodies against the toxin revealed that SEA added to apple juice appears to be largely irreversibly bound to the juice constituents. The results suggest that food-compatible and safe anti-toxin phenolic compounds can be used to inactivate SEA in vitro and possibly also in vivo, even after induction of T-cell proliferation by long-term exposure to SEA. The significance of the results for microbial food safety and human health is discussed.
Reuven Rasooly; Paula M Do; Mendel Friedman
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of agricultural and food chemistry     Volume:  58     ISSN:  1520-5118     ISO Abbreviation:  J. Agric. Food Chem.     Publication Date:  2010 May 
Date Detail:
Created Date:  2010-05-05     Completed Date:  2010-08-06     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0374755     Medline TA:  J Agric Food Chem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  5421-6     Citation Subset:  IM    
Western Regional Research Center, Agricultural Research Service, United States Department of Agriculture, Albany, California 94710, USA.
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MeSH Terms
Cell Division / drug effects
Enterotoxins / antagonists & inhibitors*
Flavonoids / pharmacology*
Malus / chemistry*
Mice, Inbred C57BL
Phenols / pharmacology*
Spleen / drug effects
Reg. No./Substance:
0/Enterotoxins; 0/Flavonoids; 0/Phenols; 0/polyphenols; 37337-57-8/enterotoxin A, Staphylococcal

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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