| Inhibition of ∆(9)-desaturase activity with sterculic acid: Effect on the endogenous synthesis of cis-9 18:1 and cis-9, trans-11 18:2 in dairy sheep. | |
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MedLine Citation:
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PMID: 22916929 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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This study was conducted in lactating ewes to examine the involvement of ∆(9)-desaturase in mammary lipogenesis, especially in the endogenous synthesis of cis-9, trans-11 18:2 and cis-9 18:1, because no information on this matter was available for dairy sheep. With this aim, 6 Assaf ewes were monitored in a 15-d experiment, which included a 5-d pretreatment period, a 5-d treatment period, and a 5-d posttreatment period. During the treatment period, ewes received 0.5g/d of sterculic acid (a cyclopropene fatty acid that inhibits ∆(9)-desaturase), delivered intravenously in 4 equal doses at 6-h intervals. Animals were fed pasture to supply mainly α-linolenic acid and minimize the amount of milk cis-9, trans-11 18:2 of ruminal origin. Sterculic acid administration was calculated to inhibit ∆(9)-desaturase by 70% based on the milk content of cis-9 14:1. This inhibition resulted in decreases in the milk content of the enzyme products (e.g., cis-9 10:1, cis-9 14:1, cis-9 16:1, cis-9 18:1, and cis-9, trans-11 18:2) and increases in its substrates (e.g., 14:0, 18:0, and trans-11 18:1), as well as in reductions in the desaturase indexes. Some other milk fatty acids, further to previously reported products or substrates of ∆(9)-desaturase (e.g., cis-15 18:1 and cis-9, cis-15 18:2, or trans-11, trans-15 18:2, and cis-9, trans-11, trans-15 18:3), were also affected by sterculic acid administration. Endogenous synthesis was the major source of cis-9 18:1 and cis-9, trans-11 18:2, accounting for 63 and 74% of its content in milk fat, respectively. To our knowledge, the present study provides the first estimates of endogenous synthesis of these 2 bioactive fatty acids in ovine milk fat. |
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Authors:
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E Bichi; P G Toral; G Hervás; P Frutos; P Gómez-Cortés; M Juárez; M A de la Fuente |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Journal of dairy science Volume: 95 ISSN: 1525-3198 ISO Abbreviation: J. Dairy Sci. Publication Date: 2012 Sep |
Date Detail:
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Created Date: 2012-08-24 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 2985126R Medline TA: J Dairy Sci Country: United States |
Other Details:
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Languages: eng Pagination: 5242-52 Citation Subset: IM |
Copyright Information:
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Copyright © 2012 American Dairy Science Association. Published by Elsevier Inc. All rights reserved. |
Affiliation:
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Instituto de Ganadería de Montaña (CSIC-ULE), Finca Marzanas s/n. 24346 Grulleros, León, Spain. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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