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Inhibition of 5-lipoxygenase and cyclooxygenase-2 pathways by pain-relieving plaster in macrophages.
MedLine Citation:
PMID:  21639685     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
Context: Pain-relieving plaster (PRP) is a traditional Chinese medicine (TCM) that has been widely used with satisfactory results in the treatment of some diseases related to inflammation, such as bruises, chronic arthritis. Objective: The mechanisms underlying the anti-inflammatory actions of PRP are investigated in this study for the first time. Materials and methods: The anti-inflammatory effects of PRP extracts were evaluated in lipopolysaccharide (LPS) or calcium ionophore A23187-treated murine peritoneal macrophages (PMs). Tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), prostaglandin E(2) (PGE(2)), and leukotrienes B(4) (LTB(4)) were evaluated by ELISA assays. Reverse transcriptase-polymerase chain reaction (RT-PCR) and western blot analysis were used to detect the expression of cyclooxygenase-2 (COX-2) and 5-lipoxygenase (5-LOX). Nuclear factor-kappa B (NF-κB)-DNA-binding activity was determined by gel mobility shift assay. Results: PRP extracts were found to inhibit the production of TNF-α, IL-1β, and PGE(2), reduce the expressions of COX-2 at the mRNA and protein levels induced by LPS, and reduced the production of LTB(4) induced by A23187. Furthermore, PRP extracts significantly attenuated LPS-induced NF-κB-DNA-binding activity. Discussion and conclusion: The anti-inflammatory effects of PRP possibly are related to reduction of inflammatory cytokines (TNF-α and IL-1β), inducible inflammatory enzyme (COX-2), and its metabolite PGE(2) via NF-κB signal pathway. Moreover, PRP extracts also notably inhibited the production of LTB(4), indicating that PRP inhibited the 5-LOX pathway, which may be the other mechanism for its anti-inflammatory action.
Authors:
Shan-Ying Peng; Yang Liu; Xu-Hong Bao; Lin Wang; Fu-Ying Zhang; Feng Wang; Wen-Jie Wang
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Pharmaceutical biology     Volume:  49     ISSN:  1744-5116     ISO Abbreviation:  Pharm Biol     Publication Date:  2011 Jul 
Date Detail:
Created Date:  2011-06-06     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9812552     Medline TA:  Pharm Biol     Country:  England    
Other Details:
Languages:  eng     Pagination:  716-26     Citation Subset:  IM    
Affiliation:
Department of pharmacology, Institute of Materia Medica, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, China.
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