| Inhibition of 5-lipoxygenase and cyclooxygenase-2 pathways by pain-relieving plaster in macrophages. | |
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MedLine Citation:
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PMID: 21639685 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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Context: Pain-relieving plaster (PRP) is a traditional Chinese medicine (TCM) that has been widely used with satisfactory results in the treatment of some diseases related to inflammation, such as bruises, chronic arthritis. Objective: The mechanisms underlying the anti-inflammatory actions of PRP are investigated in this study for the first time. Materials and methods: The anti-inflammatory effects of PRP extracts were evaluated in lipopolysaccharide (LPS) or calcium ionophore A23187-treated murine peritoneal macrophages (PMs). Tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), prostaglandin E(2) (PGE(2)), and leukotrienes B(4) (LTB(4)) were evaluated by ELISA assays. Reverse transcriptase-polymerase chain reaction (RT-PCR) and western blot analysis were used to detect the expression of cyclooxygenase-2 (COX-2) and 5-lipoxygenase (5-LOX). Nuclear factor-kappa B (NF-κB)-DNA-binding activity was determined by gel mobility shift assay. Results: PRP extracts were found to inhibit the production of TNF-α, IL-1β, and PGE(2), reduce the expressions of COX-2 at the mRNA and protein levels induced by LPS, and reduced the production of LTB(4) induced by A23187. Furthermore, PRP extracts significantly attenuated LPS-induced NF-κB-DNA-binding activity. Discussion and conclusion: The anti-inflammatory effects of PRP possibly are related to reduction of inflammatory cytokines (TNF-α and IL-1β), inducible inflammatory enzyme (COX-2), and its metabolite PGE(2) via NF-κB signal pathway. Moreover, PRP extracts also notably inhibited the production of LTB(4), indicating that PRP inhibited the 5-LOX pathway, which may be the other mechanism for its anti-inflammatory action. |
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Authors:
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Shan-Ying Peng; Yang Liu; Xu-Hong Bao; Lin Wang; Fu-Ying Zhang; Feng Wang; Wen-Jie Wang |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Pharmaceutical biology Volume: 49 ISSN: 1744-5116 ISO Abbreviation: Pharm Biol Publication Date: 2011 Jul |
Date Detail:
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Created Date: 2011-06-06 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9812552 Medline TA: Pharm Biol Country: England |
Other Details:
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Languages: eng Pagination: 716-26 Citation Subset: IM |
Affiliation:
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Department of pharmacology, Institute of Materia Medica, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, China. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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