| Inhibition of 40S--Met--tRNAfMet ribosomal initiation complex formation by vaccinia virus. | |
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MedLine Citation:
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PMID: 6968407 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Infection with vaccinia virus (a poxvirus) quickly and efficiently shuts off host protein synthesis in the presence of actinomycin D (refs 3--5) or cycloheximide. The cellular messenger RNA apparently remains stable in the infected cells exposed to inhibitors of viral gene transcription. In some cases vaccinia viral RNA or poly(A) synthesis have been implicated in the establishment of this effect. However, in the presence of cordycepin (3-deoxyadenosine) which blocks viral gene transcription and cytoplasmic poly(A) synthesis, cellular protein synthesis is still efficiently inhibited in vaccinia virus-infected cells. This shutoff is also observed in vitro, in the corresponding cell-free extracts, and in a reticulocyte lysate. Therefore the shutoff of host protein synthesis is probably mediated by a factor associated with vaccinia virions. We now report that the formation of the 40S--Met-tRNAfMet initiation complex is inhibited in cytoplasmic extracts derived from vaccinia virus-infected cells exposed to cordycepin to block viral gene expression. A similar inhibition is found in reticulocyte lysates incubated with purified vaccinia cores, confirming the hypothesis that the factor associated with the viral cores is responsible for the inhibition observed in vaccinia virus-infected cells exposed to inhibitors of transcription. |
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Authors:
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A Person; F Ben-Hamida; G Beaud |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Nature Volume: 287 ISSN: 0028-0836 ISO Abbreviation: Nature Publication Date: 1980 Sep |
Date Detail:
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Created Date: 1980-12-18 Completed Date: 1980-12-18 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0410462 Medline TA: Nature Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 355-7 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Deoxyadenosines / pharmacology Methionine Mice Peptide Chain Initiation, Translational* RNA, Transfer, Amino Acyl / metabolism Ribosomes / metabolism* Vaccinia / genetics Vaccinia virus / genetics*, ultrastructure |
| Chemical | |
Reg. No./Substance:
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0/Deoxyadenosines; 0/RNA, Transfer, Amino Acyl; 63-68-3/Methionine |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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