| Inhibition of 20-HETE attenuates diabetes-induced decreases in retinal hemodynamics. | |
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MedLine Citation:
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PMID: 21658386 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The mechanisms of early diabetes-induced decreases in retinal blood flow have yet to be fully determined. The aim of this study was to explore the hypothesis that 20-hydroxyeicosatetraenoic acid (20-HETE) plays a role in the early decrease of retinal hemodynamics in diabetic mice. 20-HETE has been implicated previously in the diabetes-enhanced vasoconstriction of mesenteric and renal vessels; however, its role in the diabetic retinal microcirculation has not been investigated. Diabetes was induced by multiple low-dose injections of streptozotocin (STZ; 50 mg/kg for 5 consecutive days), then ∼2 weeks later the mice were administered daily intraperitoneal injections with or without the 20-HETE inhibitor HET0016 (2.5 mg/kg/day) for the following 2 weeks. Non-diabetic age-matched mice were included as controls. Intravital microscopy was used to obtain measurements of retinal vascular diameters and red blood cell (RBC) velocities for the feed arterioles and draining venules extending out of and into the optic disk. From these values, wall shear rates and blood flow rates were calculated. Diabetes induced approximately 30-40% decreases in RBC velocity, wall shear rate, and blood flow rate. These decreases were attenuated to 5-10% in the mice given HET0016. In summary, the 20-HETE inhibitor HET0016 is able to attenuate the retinal hemodynamic changes induced by diabetes. |
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Authors:
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Zhongli Wang; Amit Singh Yadav; Wendy Leskova; Norman R Harris |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural Date: 2011-05-30 |
Journal Detail:
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Title: Experimental eye research Volume: 93 ISSN: 1096-0007 ISO Abbreviation: Exp. Eye Res. Publication Date: 2011 Jul |
Date Detail:
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Created Date: 2011-07-18 Completed Date: 2011-09-09 Revised Date: 2011-09-26 |
Medline Journal Info:
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Nlm Unique ID: 0370707 Medline TA: Exp Eye Res Country: England |
Other Details:
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Languages: eng Pagination: 108-13 Citation Subset: IM |
Copyright Information:
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Copyright © 2011 Elsevier Ltd. All rights reserved. |
Affiliation:
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Department of Molecular & Cellular Physiology, Louisiana State University Health Sciences Center, Shreveport, LA 71130, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Amidines
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pharmacology* Animals Blood Flow Velocity / physiology Diabetes Mellitus, Experimental / physiopathology, prevention & control* Diabetic Retinopathy / physiopathology, prevention & control* Erythrocytes / physiology Hydroxyeicosatetraenoic Acids / antagonists & inhibitors* Injections, Intraperitoneal Male Mice Mice, Inbred C57BL Regional Blood Flow / physiology Retinal Vessels / drug effects*, physiopathology |
| Grant Support | |
ID/Acronym/Agency:
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EY017599/EY/NEI NIH HHS; R01 EY017599-04/EY/NEI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Amidines; 0/Hydroxyeicosatetraenoic Acids; 0/N-hydroxy-N'-(4-butyl-2-methylphenyl)formamidine; 79551-86-3/20-hydroxy-5,8,11,14-eicosatetraenoic acid |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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