Document Detail


Inhibition of 15-hydroxy prostaglandin dehydrogenase activity in rabbit gastric antral mucosa by 13-hydroperoxyoctadecadienoic acid.
MedLine Citation:
PMID:  7708809     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The effect of 13-hydroperoxyoctadecadienoic acid (13-HPODE), a hydroperoxy adduct of linoleic acid (LA), on the activities of prostaglandin (PG) synthesizing and catabolizing enzymes in rabbit gastric antral mucosa was examined. 13-HPODE had no effect on the synthesis of PGE2, PGF2 alpha and PGD2 from exogenous arachidonic acid in the microsomal fraction of the gastric mucosa at concentrations ranging from 5-20 microM. On the other hand, at 1-10 microM, it inhibited the activity of 15-hydroxy PG dehydrogenase (PGDH), which catalyzes the initial step of catabolism of PGs, in a dose-dependent manner. The concentration required for 50% inhibition was approximately 1 microM. Experiments utilizing LA, 13-hydroperoxyoctadecadienoic acid and Fe2+ indicated the requirement of the hydroperoxy moiety for the inhibitory effect of 13-HPODE on the PGDH activity. These results suggest that 13-HPODE has the potential to increase the levels of biologically active PGs in gastric mucosa by preventing their inactivation and may have functional effects within the stomach.
Authors:
S Sakuma; Y Fujimoto; Y Miyata; K Yamane; H Nishida; T Fujita
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Prostaglandins, leukotrienes, and essential fatty acids     Volume:  51     ISSN:  0952-3278     ISO Abbreviation:  Prostaglandins Leukot. Essent. Fatty Acids     Publication Date:  1994 Dec 
Date Detail:
Created Date:  1995-05-05     Completed Date:  1995-05-05     Revised Date:  2005-11-17    
Medline Journal Info:
Nlm Unique ID:  8802730     Medline TA:  Prostaglandins Leukot Essent Fatty Acids     Country:  SCOTLAND    
Other Details:
Languages:  eng     Pagination:  425-30     Citation Subset:  IM    
Affiliation:
Department of Hygienic Chemistry, Osaka University of Pharmaceutical Sciences, Japan.
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MeSH Terms
Descriptor/Qualifier:
Animals
Arachidonic Acid / metabolism
Chromatography, High Pressure Liquid
Cytosol / enzymology
Dinoprost / metabolism
Dinoprostone / analogs & derivatives,  metabolism
Gastric Mucosa / drug effects*,  enzymology
Hydroxyprostaglandin Dehydrogenases / antagonists & inhibitors*
Linoleic Acids / pharmacology*
Lipid Peroxides*
Male
Microsomes / enzymology
Prostaglandin D2 / metabolism
Prostaglandin-Endoperoxide Synthases / metabolism
Prostaglandins / biosynthesis*
Pyloric Antrum / drug effects*,  enzymology
Rabbits
Chemical
Reg. No./Substance:
0/Linoleic Acids; 0/Lipid Peroxides; 0/Prostaglandins; 23017-93-8/13-hydroperoxy-9,11-octadecadienoic acid; 26441-05-4/15-ketoprostaglandin E2; 363-23-5/15-keto-13,14-dihydroprostaglandin E2; 363-24-6/Dinoprostone; 41598-07-6/Prostaglandin D2; 506-32-1/Arachidonic Acid; 551-11-1/Dinoprost; EC 1.1.1.-/Hydroxyprostaglandin Dehydrogenases; EC 1.1.1.141/15-hydroxyprostaglandin dehydrogenase; EC 1.14.99.1/Prostaglandin-Endoperoxide Synthases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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