Document Detail

Inhibiting inducible nitric oxide synthase with rutin reduces renal ischemia/reperfusion injury.
MedLine Citation:
PMID:  23187035     Owner:  NLM     Status:  MEDLINE    
BACKGROUND: Nitric oxide (NO) seems to play an important role during renal ischemia/reperfusion (I/R) injury. We investigated whether rutin inhibits inducible nitric oxide synthase (iNOS) and reduces 3-nitrotyrosine (3-NT) formation in the kidneys of rats during I/R.
METHODS: Wistar albino rats were nephrectomized unilaterally and, 2 weeks later, subjected to 45 minutes of left renal pedicle occlusion followed by 3 hours of reperfusion. We intraperitoneally administered L-N6-(1-iminoethyl)lysine (L-NIL; 3 mg/kg) for 30 minutes or rutin (1 g/kg) for 60 minutes before I/R. After reperfusion, kidney samples were taken for immunohistochemical analysis of iNOS and 3-NT. We measured plasma nitrite/nitrate and cyclic guanosine monophosphate (cGMP) to evaluate NO levels.
RESULTS: Ischemia/reperfusion caused plasma cGMP to increase significantly. Similarly, plasma nitrite/nitrate was elevated in the I/R group compared with the control group. Histochemical staining was positive for iNOS and 3-NT in the I/R group. Pretreatment with L-NIL or rutin significantly mitigated the elevation of plasma cGMP and nitrite/nitrate. These changes in biochemical parameters were also associated with changes in immunohistochemical appearance. Pretreatment with L-NIL or rutin significantly decreased the incidence and severity of iNOS and 3-NT formation in the kidney tissues.
CONCLUSION: Our findings suggest that high activity of iNOS causes renal I/R injury, and that rutin exerts protective effects, probably by inhibiting iNOS.
Asli Korkmaz; Dürdane Kolankaya
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Canadian journal of surgery. Journal canadien de chirurgie     Volume:  56     ISSN:  1488-2310     ISO Abbreviation:  Can J Surg     Publication Date:  2013 Feb 
Date Detail:
Created Date:  2013-01-28     Completed Date:  2013-03-17     Revised Date:  2013-07-11    
Medline Journal Info:
Nlm Unique ID:  0372715     Medline TA:  Can J Surg     Country:  Canada    
Other Details:
Languages:  eng     Pagination:  6-14     Citation Subset:  IM    
The Ministry of Agriculture and Rural Affairs, National Food Reference Laboratory, Yenimahalle, Turkey.
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MeSH Terms
Biological Markers / blood
Cyclic GMP / blood
Enzyme Inhibitors / administration & dosage,  pharmacology*
Infusions, Parenteral
Kidney / drug effects*,  enzymology,  metabolism
Lysine / analogs & derivatives,  pharmacology
Nitrates / blood
Nitric Oxide Synthase Type II / antagonists & inhibitors*,  metabolism
Nitrites / blood
Protective Agents / administration & dosage,  pharmacology*
Rats, Wistar
Reperfusion Injury / enzymology,  metabolism*,  prevention & control*
Rutin / administration & dosage,  pharmacology*
Tyrosine / analogs & derivatives*,  drug effects,  metabolism
Reg. No./Substance:
0/Biological Markers; 0/Enzyme Inhibitors; 0/N(6)-(1-iminoethyl)lysine; 0/Nitrates; 0/Nitrites; 0/Protective Agents; 153-18-4/Rutin; 3604-79-3/3-nitrotyrosine; 55520-40-6/Tyrosine; 56-87-1/Lysine; 7665-99-8/Cyclic GMP; EC Oxide Synthase Type II

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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