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Inhibiting effect of the new cytotoxic antibiotic daunomycin on nucleic acids and mitotic activity of HeLa cells.
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MedLine Citation:
PMID:  5885429     Owner:  NLM     Status:  MEDLINE    
The effect has been studied of Actinomycin D, Daunomycin (Da.), and Da. N acetyl derivative on mitotic activity and on the nucleic acid synthesis of in vitro HeLa cell cultures. The experiments were carried out by means of the radioautographic technique using stripping films. The relative uptake of thymidine-H(3) and uridine-H(3) was determined by means of the reduced silver grain count present in the nuclei of controls and treated cells. The mitotic activity and thymidine incorporation were noticeably reduced by Daunomycin and Actinomycin, whereas both processes appeared less affected by Da. N acetyl derivative. As regards nuclear RNA synthesis, all three antibiotics at low doses chiefly inhibit nucleolar RNA synthesis. On the other hand, whilst Actinomycin at higher doses causes an almost total inhibition of the synthesis of the whole nuclear RNA, in Daunomycin- and Da. N acetyl derivative-treated cells extranucleolar RNA synthesis is less susceptible to inhibition.
A Di Marco; R Silvestrini; S Di Marco; T Dasdia
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Publication Detail:
Type:  In Vitro; Journal Article    
Journal Detail:
Title:  The Journal of cell biology     Volume:  27     ISSN:  0021-9525     ISO Abbreviation:  J. Cell Biol.     Publication Date:  1965 Dec 
Date Detail:
Created Date:  1966-09-25     Completed Date:  1966-09-25     Revised Date:  2010-06-22    
Medline Journal Info:
Nlm Unique ID:  0375356     Medline TA:  J Cell Biol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  545-50     Citation Subset:  IM    
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MeSH Terms
Cell Division / drug effects*
Culture Techniques*
DNA / biosynthesis*
Daunorubicin / pharmacology*
Hela Cells
RNA / biosynthesis*
Thymidine / metabolism
Uridine / metabolism
Reg. No./Substance:
20830-81-3/Daunorubicin; 50-89-5/Thymidine; 58-96-8/Uridine; 63231-63-0/RNA; 9007-49-2/DNA

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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Journal Information
Journal ID (nlm-ta): J Cell Biol
ISSN: 0021-9525
ISSN: 1540-8140
Publisher: The Rockefeller University Press
Article Information
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Received Day: 23 Month: 3 Year: 1965
Print publication date: Day: 1 Month: 12 Year: 1965
Volume: 27 Issue: 3
First Page: 545 Last Page: 550
ID: 2106764
PubMed Id: 5885429

A. Di Marco
R. Silvestrini
S. Di Marco
T. Dasdia
From Gruppo di Ricerche Oncologiche del Consiglio Nazionale delle Ricerche, Istituto Nazionale per lo Studio e la Cura dei Tumori, Sezione di Chemioterapia Sperimentale, Milano, Italy

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