Document Detail


Inhibin, activin, follistatin, activin receptors and beta-glycan gene expression in the villous tissue of miscarriage patients.
MedLine Citation:
PMID:  15361555     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Maternal circulating levels of inhibin A are significantly lower in patients with clinical symptoms of miscarriage. The objective of this study was to quantify relative expression of inhibin alpha, inhibin/activin betaA, betaB, betaC, follistatin, activin receptors and beta-glycan genes and content of inhibin A, activin A and follistatin protein in villous tissue of first trimester miscarriages and gestation-matched normal pregnancies. Twelve women with clinical symptoms of miscarriage were matched with 12 normal pregnancies for gestational age. Total RNA was isolated from placental samples. Complementary DNA produced by reverse transcription was used in the real-time PCR to quantify the expression of the genes. The ratio between the target and rRNA 18S was calculated to provide relative gene expression. Villous tissue homogenates were used for the determination of the content of inhibin A, activin A and follistatin protein. Maternal serum was assayed for inhibin A, activin A and follistatin. All villous samples expressed inhibin alpha, inhibin/activin betaA, betaB, betaC, follistatin, activin receptors (ACTRIA, ACTRIB, ACTRIIA, ACTRIIB) and beta-glycan genes. There was no significant difference in the relative expression of these genes between the groups. Villous content of inhibin A, activin A and follistatin were also not different between the two groups. Maternal serum levels of inhibin A were significantly lower in the miscarriage group compared to the controls. The decreased maternal levels of inhibin A in miscarriage patients could be due to a decrease in placental mass prior to embryonic demise. This finding also confirms that the trophoblast is the major source of inhibin A after the luteo-placental shift in early pregnancy.
Authors:
S Muttukrishna; C Bearfield; J Johns; E Jauniaux
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't     Date:  2004-09-10
Journal Detail:
Title:  Molecular human reproduction     Volume:  10     ISSN:  1360-9947     ISO Abbreviation:  Mol. Hum. Reprod.     Publication Date:  2004 Nov 
Date Detail:
Created Date:  2004-10-20     Completed Date:  2005-06-02     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  9513710     Medline TA:  Mol Hum Reprod     Country:  England    
Other Details:
Languages:  eng     Pagination:  793-8     Citation Subset:  IM    
Affiliation:
Department of Obstetrics and Gynaecology, Royal Free University College Medical School, 86-96 Chenies Mews, London WC1E 6HX, UK. s.muttukrishna@ucl.ac.uk
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MeSH Terms
Descriptor/Qualifier:
Abortion, Spontaneous / genetics,  metabolism*
Activin Receptors / analysis,  genetics,  metabolism*
Activins / analysis,  genetics,  metabolism
Adult
Chorionic Villi / immunology,  metabolism*
Female
Follistatin / analysis,  genetics,  metabolism
Gene Expression
Gonadal Hormones / genetics,  metabolism*
Humans
Inhibins / analysis,  genetics,  metabolism
Pregnancy
Proteoglycans / genetics,  metabolism*
RNA, Messenger / analysis,  metabolism
Receptors, Transforming Growth Factor beta / genetics,  metabolism*
Chemical
Reg. No./Substance:
0/Follistatin; 0/Gonadal Hormones; 0/Proteoglycans; 0/RNA, Messenger; 0/Receptors, Transforming Growth Factor beta; 104625-48-1/Activins; 145170-29-2/betaglycan; 57285-09-3/Inhibins; EC 2.7.11.30/Activin Receptors

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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