Document Detail


Inherited effects of low-dose exposure to methylmercury in neural stem cells.
MedLine Citation:
PMID:  22918959     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Methylmercury (MeHg) is an environmental contaminant with recognized neurotoxic effects, particularly to the developing nervous system. In the present study we show that nanomolar concentrations of MeHg can induce long-lasting effects in neural stem cells (NSCs). We investigated short-term direct and long-term inherited effects of exposure to MeHg (2.5 nM or 5.0 nM) using primary cultures of rat embryonic cortical NSCs. We found that MeHg had no adverse effect on cell viability but reduced NSC proliferation and altered the expression of cell cycle regulators (p16 and p21) and senescence-associated markers. In addition, we demonstrated a decrease in global DNA methylation in the exposed cells, indicating that epigenetic changes may be involved in the mechanisms underlying MeHg-induced effects. These changes were observed in cells directly exposed to MeHg (parent cells) and in their daughter cells cultured under MeHg-free conditions. In agreement with our in vitro data, a trend was found for decreased cell proliferation in the subgranular zone in the hippocampi of adult mice exposed to low doses of MeHg during the perinatal period. Interestingly, this impaired proliferation had a measurable impact on the total number of neurons in the hippocampal dentate gyrus. Importantly, this effect could be reversed by chronic antidepressant treatment. Our study provides novel evidence for programming effects induced by MeHg in NSCs and supports the idea that developmental exposure to low levels of MeHg may result in long-term consequences predisposing to neurodevelopmental disorders and/or neurodegeneration.
Authors:
Raj Bose; Natalia Onishchenko; Karin Edoff; Ann Marie Janson Lang; Sandra Ceccatelli
Related Documents :
6112049 - The membrane potential of cat hippocampal neurons recorded in vivo displays four differ...
21769329 - Targeted ph-dependent fluorescent activity-based cathepsin probes.
1864339 - Orientation bias of cat dorsal lateral geniculate cells: directional analysis of the ma...
18287499 - Complementary modulation of somatic inhibition by opioids and cannabinoids.
21636699 - Molecular and cellular pharmacology of the novel noncamptothecin topoisomerase i inhibi...
21775119 - The effects of transformation and znt-1 silencing on zinc homeostasis in cultured cells.
3161769 - Structure, function, and immune properties of reassociated islet cells.
1379219 - Reversion of radiosensitivity in azacytidine-treated xrs5 cells does not result in full...
21701989 - Rita levi-montalcini and the discovery of ngf, the first nerve cell growth factor.
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-8-23
Journal Detail:
Title:  Toxicological sciences : an official journal of the Society of Toxicology     Volume:  -     ISSN:  1096-0929     ISO Abbreviation:  Toxicol. Sci.     Publication Date:  2012 Aug 
Date Detail:
Created Date:  2012-8-24     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9805461     Medline TA:  Toxicol Sci     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
Department of Neuroscience, Karolinska Institutet, Retzius väg 8, 17177 Stockholm, Sweden.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Pkh1/2-dependent phosphorylation of Vps27 regulates ESCRT-I recruitment to endosomes.
Next Document:  Molecular reconstruction of extinct LINE-1 elements and their interaction with nonautonomous element...