Document Detail


Inherent radiosensitivity and split-dose recovery in plateau-phase cultures of 10 human tumour cell lines.
MedLine Citation:
PMID:  1496140     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The radiation response of 10 human tumour cell lines, eight of them established in our Institute, was analysed. Single dose acute survival curves were constructed and fitted with the linear-quadratic (LQ) model. The mean inactivation dose (D) was also calculated, together with D(o) and n. In order to measure both split-dose recovery and delayed plating recovery of plateau-phase cells, confluent cultures were subjected to two doses of 2 Gy 24 h apart, and plated 24 h later, simulating clinical fractionation. Survival of these cells (S2 x 2) was compared to that following 4 Gy given to cells plated at low density and an overall recovery factor (RF) was derived, including both types of recovery. S2 x 2 and RF were compared to the intrinsic radiosensitivity parameters. The three melanoma cell lines differed in radiation response, and the three squamous cell carcinoma cell lines were rather radioresistant. The neuroblastoma cell line was highly sensitive, yet it expressed the highest beta and the highest RF. Using a non-parametric correlation test, S2 x 2 was found to be related to D, whereas RF was not related to the radiosensitivity parameters. However, the two cell lines with the lowest D and the lowest S2 expressed the highest RF. These results suggest that radiosensitive cell lines may have a considerable capacity to recover if confluent cultures are exposed to fractionated irradiation. The overall recovery factor (RF) used here is proposed as a useful measure of cellular recovery.
Authors:
A Courdi; R J Bensadoun; J Gioanni; C Caldani
Publication Detail:
Type:  In Vitro; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Radiotherapy and oncology : journal of the European Society for Therapeutic Radiology and Oncology     Volume:  24     ISSN:  0167-8140     ISO Abbreviation:  Radiother Oncol     Publication Date:  1992 Jun 
Date Detail:
Created Date:  1992-09-10     Completed Date:  1992-09-10     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8407192     Medline TA:  Radiother Oncol     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  102-7     Citation Subset:  IM    
Affiliation:
Department of Radiotherapy, Centre Antoine-Lacassagne, Nice, France.
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MeSH Terms
Descriptor/Qualifier:
Cell Survival / radiation effects
Dose-Response Relationship, Radiation
Humans
Radiation Tolerance*
Tumor Cells, Cultured / radiation effects*

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