Document Detail


Inhaled nitric oxide inhibits the release of matrix metalloproteinase-2, but not platelet activation, during extracorporeal membrane oxygenation in adult rabbits.
MedLine Citation:
PMID:  12677560     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND/PURPOSE: In neonates receiving extracorporeal membrane oxygenation (ECMO), platelet activation and dysfunction occur with the release of matrix metalloproteinase (MMP)-2, which stimulates platelet aggregation. Because inhaled nitric oxide (NO) reduces pulmonary hypertension and inhibits platelet aggregation, the authors examined the effects of inhaled NO on platelet activation induced by ECMO. METHODS: Ten adult white New Zealand rabbits were instrumented for ECMO and assigned randomly to receive either inhaled NO at 40 ppm or 30% oxygen for 1 hour before ECMO and continued for 4 hours after starting ECMO. Platelet counts, collagen-induced platelet aggregation ex vivo, plasma MMP-2, and MMP-9 activities were measured. RESULTS: (1) ECMO caused thrombocytopenia, decreased platelet aggregation, and increased plasma MMP-2 and MMP-9 activities in controls. (2) Inhaled NO inhibited platelet aggregation before ECMO but did not affect the ECMO-induced thrombocytopenia and platelet activation. (3) Inhaled NO significantly abolished the ECMO-induced increase in plasma MMP-2 but not MMP-9 activities. CONCLUSIONS: Although inhaled NO did not inhibit the platelet activation during ECMO in adult rabbits, it attenuated the increase in plasma MMP-2 activity that may be important for neonates treated with ECMO.
Authors:
P-Y Cheung; G Sawicki; A Peliowski; P C Etches; R Schulz; M W Radomski
Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of pediatric surgery     Volume:  38     ISSN:  1531-5037     ISO Abbreviation:  J. Pediatr. Surg.     Publication Date:  2003 Apr 
Date Detail:
Created Date:  2003-04-04     Completed Date:  2003-11-12     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  0052631     Medline TA:  J Pediatr Surg     Country:  United States    
Other Details:
Languages:  eng     Pagination:  534-8     Citation Subset:  IM    
Copyright Information:
Copyright 2003, Elsevier Science (USA). All rights reserved.
Affiliation:
Department of Pediatrics, University of Alberta, Edmonton, Alberta, Canada.
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MeSH Terms
Descriptor/Qualifier:
Administration, Inhalation
Animals
Blood Platelets / drug effects,  secretion,  ultrastructure
Carbon Dioxide / blood
Disease Models, Animal
Drug Evaluation, Preclinical
Extracorporeal Membrane Oxygenation* / adverse effects
Female
Hemodynamics / drug effects
Matrix Metalloproteinase 2 / secretion*
Matrix Metalloproteinase 9 / secretion
Nitric Oxide / administration & dosage,  pharmacology*,  therapeutic use
Oxygen / blood,  pharmacology
Partial Pressure
Platelet Activation / drug effects*
Platelet Aggregation / drug effects
Rabbits
Respiratory Insufficiency / therapy
Thrombocytopenia / blood,  etiology
Chemical
Reg. No./Substance:
10102-43-9/Nitric Oxide; 124-38-9/Carbon Dioxide; 7782-44-7/Oxygen; EC 3.4.24.24/Matrix Metalloproteinase 2; EC 3.4.24.35/Matrix Metalloproteinase 9

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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