| Inhaled nitric oxide inhibits the release of matrix metalloproteinase-2, but not platelet activation, during extracorporeal membrane oxygenation in adult rabbits. | |
MedLine Citation:
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PMID: 12677560 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND/PURPOSE: In neonates receiving extracorporeal membrane oxygenation (ECMO), platelet activation and dysfunction occur with the release of matrix metalloproteinase (MMP)-2, which stimulates platelet aggregation. Because inhaled nitric oxide (NO) reduces pulmonary hypertension and inhibits platelet aggregation, the authors examined the effects of inhaled NO on platelet activation induced by ECMO. METHODS: Ten adult white New Zealand rabbits were instrumented for ECMO and assigned randomly to receive either inhaled NO at 40 ppm or 30% oxygen for 1 hour before ECMO and continued for 4 hours after starting ECMO. Platelet counts, collagen-induced platelet aggregation ex vivo, plasma MMP-2, and MMP-9 activities were measured. RESULTS: (1) ECMO caused thrombocytopenia, decreased platelet aggregation, and increased plasma MMP-2 and MMP-9 activities in controls. (2) Inhaled NO inhibited platelet aggregation before ECMO but did not affect the ECMO-induced thrombocytopenia and platelet activation. (3) Inhaled NO significantly abolished the ECMO-induced increase in plasma MMP-2 but not MMP-9 activities. CONCLUSIONS: Although inhaled NO did not inhibit the platelet activation during ECMO in adult rabbits, it attenuated the increase in plasma MMP-2 activity that may be important for neonates treated with ECMO. |
Authors:
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P-Y Cheung; G Sawicki; A Peliowski; P C Etches; R Schulz; M W Radomski |
Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of pediatric surgery Volume: 38 ISSN: 1531-5037 ISO Abbreviation: J. Pediatr. Surg. Publication Date: 2003 Apr |
Date Detail:
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Created Date: 2003-04-04 Completed Date: 2003-11-12 Revised Date: 2007-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0052631 Medline TA: J Pediatr Surg Country: United States |
Other Details:
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Languages: eng Pagination: 534-8 Citation Subset: IM |
Copyright Information:
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Copyright 2003, Elsevier Science (USA). All rights reserved. |
Affiliation:
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Department of Pediatrics, University of Alberta, Edmonton, Alberta, Canada. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Administration, Inhalation Animals Blood Platelets / drug effects, secretion, ultrastructure Carbon Dioxide / blood Disease Models, Animal Drug Evaluation, Preclinical Extracorporeal Membrane Oxygenation* / adverse effects Female Hemodynamics / drug effects Matrix Metalloproteinase 2 / secretion* Matrix Metalloproteinase 9 / secretion Nitric Oxide / administration & dosage, pharmacology*, therapeutic use Oxygen / blood, pharmacology Partial Pressure Platelet Activation / drug effects* Platelet Aggregation / drug effects Rabbits Respiratory Insufficiency / therapy Thrombocytopenia / blood, etiology |
| Chemical | |
Reg. No./Substance:
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10102-43-9/Nitric Oxide; 124-38-9/Carbon Dioxide; 7782-44-7/Oxygen; EC 3.4.24.24/Matrix Metalloproteinase 2; EC 3.4.24.35/Matrix Metalloproteinase 9 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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