| Inhaled marijuana smoke disrupts mitochondrial energetics in pulmonary epithelial cells in vivo. | |
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MedLine Citation:
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PMID: 16414979 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Habitual marijuana smoking is associated with inflammation and atypia of airway epithelium accompanied by symptoms of chronic bronchitis. We hypothesized that Delta(9)-tetrahydrocannabinol (THC), the primary psychoactive component of marijuana, might contribute to these findings by impairing cellular energetics and mitochondrial function. To test this hypothesis, we examined particulate smoke extracts from marijuana cigarettes, tobacco cigarettes, and placebo marijuana (0% THC) cigarettes for their effects on the mitochondrial function of A549 cells in vitro. Only extracts prepared from marijuana cigarettes altered mitochondrial staining by the potentiometric probe JC-1. With the use of a cross-flow, nose-only inhalation system, rats were then exposed for 20 min to whole marijuana smoke and examined for its effects on airway epithelial cells. Inhalation of marijuana smoke produced lung tissue concentrations of THC that were 8-10 times higher than those measured in blood (75 +/- 38 ng/g wet wt tissue vs. 9.2 +/- 2.0 ng/ml), suggesting high local exposure. Intratracheal infusion of JC-1 immediately following marijuana smoke exposure revealed a diffuse decrease in lung cell JC-1 red fluorescence compared with tissue from unexposed or placebo smoke-exposed rats. Exposure to marijuana smoke in vivo also decreased JC-1 red fluorescence (54% decrease, P < 0.01) and ATP levels (75% decrease, P < 0.01) in single-cell preparations of tracheal epithelial cells. These results suggest that inhalation of marijuana smoke has deleterious effects on airway epithelial cell energetics that may contribute to the adverse pulmonary consequences of marijuana smoking. |
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Authors:
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Theodore A Sarafian; Nancy Habib; Michael Oldham; Navindra Seeram; Ru-Po Lee; Laura Lin; Donald P Tashkin; Michael D Roth |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural Date: 2006-01-13 |
Journal Detail:
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Title: American journal of physiology. Lung cellular and molecular physiology Volume: 290 ISSN: 1040-0605 ISO Abbreviation: Am. J. Physiol. Lung Cell Mol. Physiol. Publication Date: 2006 Jun |
Date Detail:
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Created Date: 2006-05-10 Completed Date: 2006-08-11 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 100901229 Medline TA: Am J Physiol Lung Cell Mol Physiol Country: United States |
Other Details:
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Languages: eng Pagination: L1202-9 Citation Subset: IM |
Affiliation:
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Division of Pulmonary and Critical Care, Department of Medicine, David Geffen School of Medicine at UCLA, Los Angeles, CA 90095-1690, USA. tsarafian@mednet.ucla.edu |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cell Line Energy Metabolism Humans Intracellular Membranes / drug effects, physiology Male Marijuana Smoking / adverse effects* Membrane Potentials / drug effects, physiology Mitochondria / drug effects, physiology* Rats Rats, Sprague-Dawley Respiratory Mucosa / drug effects, physiology, physiopathology* Smoking Tetrahydrocannabinol / pharmacology |
| Grant Support | |
ID/Acronym/Agency:
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R37 DA 03018-23/DA/NIDA NIH HHS |
| Chemical | |
Reg. No./Substance:
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1972-08-3/Tetrahydrocannabinol |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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