Document Detail


Inhaled lysophosphatidylcholine provokes bronchoconstriction in guinea pigs in vivo.
MedLine Citation:
PMID:  16150438     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Lysophosphatidylcholine is increased in the airway of bronchial asthma, but its role is not clear. We investigated the role of lysophosphatidylcholine in asthma in anaesthetized, mechanically ventilated guinea pigs. Pressure at the airway opening was measured as an index of bronchial response. Increasing doses of lysophosphatidylcholine (1--10 mg/ml) were inhaled and then bronchoalveolar lavage was carried out. 100 and 200 microg/ml methacholine were inhaled 10 min after inhalation of 2.5 mg/ml lysophosphatidylcholine, 10 mg/ml dipalmitoyl phosphatidylcholine and 10 mg/ml glycerophosphocholine, all of which per se did not change the pressure at the airway opening. Effect of 1.0 microg/kg salbutamol, or 60 mg/kg diphenhydramine on the lysophosphatidylcholine-induced increase in the pressure at the airway opening was investigated. Inhalation of lysophosphatidylcholine dose-dependently increased the pressure at the airway opening and increased bronchial responsiveness to methacholine. On the other hand, inhalation of dipalmitoyl phosphatidylcholine decreased the pressure at the airway opening and decreased bronchial responsiveness to methacholine. Intravenously administered salbutamol, but not diphenhydramine, prevented the lysophosphatidylcholine-induced increase in the pressure at the airway opening. The percentage of leukocytes in bronchoalveolar lavage fluid did not change significantly at least within 20 min after the lysophosphatidylcholine inhalation. Lysophosphatidylcholine causes bronchoconstriction and enhances bronchial responsiveness without inducing leukocyte infiltration in the airway, suggesting that lysophosphatidylcholine may be a new bronchoconstrictor mediator in bronchial asthma.
Authors:
Kouichi Nobata; Kazuyoshi Kurashima; Masaki Fujimura; Miki Abo; Yoshihisa Ishiura; Kazuo Kasahara; Shinji Nakao
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  European journal of pharmacology     Volume:  520     ISSN:  0014-2999     ISO Abbreviation:  Eur. J. Pharmacol.     Publication Date:  2005 Sep 
Date Detail:
Created Date:  2005-09-27     Completed Date:  2006-02-10     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  1254354     Medline TA:  Eur J Pharmacol     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  150-5     Citation Subset:  IM    
Affiliation:
Respiratory Medicine, Cellular Transplantation Biology, Kanazawa University Graduate School of Medicine, 13-1 Takara-machi, Kanazawa 920-8641, Japan. k-nobata@yg7.so-net.ne.jp
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MeSH Terms
Descriptor/Qualifier:
1,2-Dipalmitoylphosphatidylcholine / pharmacology
Administration, Inhalation
Albuterol
Animals
Bronchial Hyperreactivity / etiology,  physiopathology*
Bronchoconstriction*
Bronchoconstrictor Agents / administration & dosage,  pharmacology*
Bronchodilator Agents
Guinea Pigs
Lysophosphatidylcholines / administration & dosage,  pharmacology*
Male
Methacholine Chloride / administration & dosage,  pharmacology
Chemical
Reg. No./Substance:
0/Bronchoconstrictor Agents; 0/Bronchodilator Agents; 0/Lysophosphatidylcholines; 18559-94-9/Albuterol; 2644-64-6/1,2-Dipalmitoylphosphatidylcholine; 62-51-1/Methacholine Chloride

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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