Document Detail

Inhaled endotoxin in healthy human subjects: a dose-related study on systemic effects and peripheral CD4+ and CD8+ T cells.
MedLine Citation:
PMID:  16039108     Owner:  NLM     Status:  MEDLINE    
BACKGROUND: Inhaled endotoxin or lipopolysaccharide (LPS) is implicated in the pathogenesis of pulmonary diseases. We investigated the inhalation effects of two different doses of LPS in healthy human subjects. METHODS: Eighteen healthy non-atopic human subjects inhaled either 15 microg (n=10) or 50 microg (n=8)Escherichia coli LPS in an open study. As control, each subject had isotonic saline inhalation 1 week before (baseline) and after LPS inhalation. Data collected included those of clinical parameter, induced sputum and peripheral blood CD4+ and CD8+ T cells. RESULTS: Acute flu-like symptoms and pyrexia were significantly greater in the 50 microg than 15 microg LPS group. Similarly, the increase in sputum and blood total cell and neutrophil counts at 6h following inhaled LPS were greater in the 50 microg group. Myeloperoxidase, human neutrophil elastase and interleukin-8 in sputum sol, but not blood, showed a trend towards greater increase following 50 microg LPS. All these changes were resolved at one week. In the 50 microg dose group alone, there was a reduction in the proportion of peripheral blood interferon (IFN)-gamma-producing CD4+ and CD8+ T cells at 6h followed by an increase at 1 week after inhaled LPS. CONCLUSIONS: The airway and systemic effects of inhaled LPS are dose-related and predominantly neutrophilic. The changes in the proportions of circulating CD4+ and CD8+ T cells suggests preferential recruitment of IFN-gamma-producing T cells into tissue from inhaled 50 microg LPS, followed by reappearance of these cells in blood 1 week later.
L C Loh; B Vyas; V Kanabar; D M Kemeny; B J O'Connor
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Publication Detail:
Type:  Journal Article     Date:  2005-07-20
Journal Detail:
Title:  Respiratory medicine     Volume:  100     ISSN:  0954-6111     ISO Abbreviation:  Respir Med     Publication Date:  2006 Mar 
Date Detail:
Created Date:  2006-02-15     Completed Date:  2006-08-29     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8908438     Medline TA:  Respir Med     Country:  England    
Other Details:
Languages:  eng     Pagination:  519-28     Citation Subset:  IM    
Department of Medicine, Clinical School, International Medical University, Jalan Rasah, Seremban 70300, Negeri Sembilan, Malaysia.
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MeSH Terms
Administration, Inhalation
CD4-Positive T-Lymphocytes / drug effects*,  metabolism
CD8-Positive T-Lymphocytes / drug effects*,  metabolism
Dose-Response Relationship, Drug
Lipopolysaccharides / administration & dosage,  adverse effects*
Lymphocyte Count
Sputum / cytology
Reg. No./Substance:

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