Document Detail

Ingestion and survival of Y. pseudotuberculosis in HeLa cells.
MedLine Citation:
PMID:  766929     Owner:  NLM     Status:  MEDLINE    
HeLa cells were infected with Yersinia pseudotuberculosis for 0.5-3 h. Intracellular bacteria could then be demonstrated by three different techniques: viable count, fluorescent-antibody staining and electron microscopy. Most of the bacteria seemed to be viable, since there was a good positive correlation (0.94) between viable and fluorescent bacteria. The bacterial uptake seemed to be mediated by a phagocytic-like procedure. The intracellular bacteria seemed to reside in vacuoles some of which increased in size as a function of time. The kinetics of infection was studied after addition of 10(7) or 10(9) bacteria per cell culture (2 X 10(6) cells). After a lag period of about 30 min there was a linear increase of intracellular bacteria, and this uptake proceeded for 1-2 h until most of the bacteria were ingested or an upper limit of ingested bacteria was reached. The upper limit was calculated to be a mean of 60 per infected cell in the cell culture. More than 90% of the cells could be infected and a reasonable number of the bacteria survive in the cells for at least 3 days, as demonstrated by the viable-count technique. The bacteria-cell system may be used to study, for example, the effect of antibiotics or antibodies on intracellular bacteria and pathogenicity of intracellular diseases.
A Bovallius; G Nilsson
Related Documents :
20977569 - Exploitation of the ubiquitin system by invading bacteria.
9860139 - Control of the human cell cycle by a bacterial protein, gapstatin.
8953049 - Requirement of cdc42 for salmonella-induced cytoskeletal and nuclear responses.
8459779 - Copper accumulation by a strain of pseudomonas putida.
3148609 - Quantitative evaluation of bacteria washed from stainless steel penicylinders during ao...
330879 - Genetic and physiological control of host cell lysis by bacteriophage lambda.
20719279 - Live cell-imaging techniques for analyses of microtubules in dictyostelium.
23244389 - Pax4 is not essential for beta-cell differentiation in zebrafish embryos but modulates ...
3435549 - Polyvalent inhibitory action of fusidic acid in isolated rat liver cells.
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Canadian journal of microbiology     Volume:  21     ISSN:  0008-4166     ISO Abbreviation:  Can. J. Microbiol.     Publication Date:  1975 Dec 
Date Detail:
Created Date:  1976-06-02     Completed Date:  1976-06-02     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  0372707     Medline TA:  Can J Microbiol     Country:  CANADA    
Other Details:
Languages:  eng     Pagination:  1997-2007     Citation Subset:  IM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Cytoplasm / microbiology
Hela Cells / immunology,  microbiology*
Pasteurella Infections / microbiology*
Vacuoles / microbiology
Yersinia* / immunology
Yersinia pseudotuberculosis Infections / microbiology*

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  The survival of Escherichia coli from freeze-thaw damage: the relative importance of wall and membra...
Next Document:  Purification and characterization of tuberculin-active components from BCG.