Document Detail


Ingestion and survival of Y. pseudotuberculosis in HeLa cells.
MedLine Citation:
PMID:  766929     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
HeLa cells were infected with Yersinia pseudotuberculosis for 0.5-3 h. Intracellular bacteria could then be demonstrated by three different techniques: viable count, fluorescent-antibody staining and electron microscopy. Most of the bacteria seemed to be viable, since there was a good positive correlation (0.94) between viable and fluorescent bacteria. The bacterial uptake seemed to be mediated by a phagocytic-like procedure. The intracellular bacteria seemed to reside in vacuoles some of which increased in size as a function of time. The kinetics of infection was studied after addition of 10(7) or 10(9) bacteria per cell culture (2 X 10(6) cells). After a lag period of about 30 min there was a linear increase of intracellular bacteria, and this uptake proceeded for 1-2 h until most of the bacteria were ingested or an upper limit of ingested bacteria was reached. The upper limit was calculated to be a mean of 60 per infected cell in the cell culture. More than 90% of the cells could be infected and a reasonable number of the bacteria survive in the cells for at least 3 days, as demonstrated by the viable-count technique. The bacteria-cell system may be used to study, for example, the effect of antibiotics or antibodies on intracellular bacteria and pathogenicity of intracellular diseases.
Authors:
A Bovallius; G Nilsson
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Canadian journal of microbiology     Volume:  21     ISSN:  0008-4166     ISO Abbreviation:  Can. J. Microbiol.     Publication Date:  1975 Dec 
Date Detail:
Created Date:  1976-06-02     Completed Date:  1976-06-02     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  0372707     Medline TA:  Can J Microbiol     Country:  CANADA    
Other Details:
Languages:  eng     Pagination:  1997-2007     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Cytoplasm / microbiology
Hela Cells / immunology,  microbiology*
Humans
Pasteurella Infections / microbiology*
Vacuoles / microbiology
Yersinia* / immunology
Yersinia pseudotuberculosis Infections / microbiology*

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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