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Infusion with the antioxidant N-acetylcysteine attenuates early adaptive responses to exercise in human skeletal muscle.
MedLine Citation:
PMID:  21827635     Owner:  NLM     Status:  Publisher    
Aim:  Production of reactive oxygen species (ROS) in skeletal muscle is markedly increased during exercise and may be essential for exercise adaptation. We therefore investigated the effects of infusion with the antioxidant N-acetylcysteine (NAC) on exercise-induced activation of signalling pathways and genes involved in exercise adaptation in human skeletal muscle. Methods:  Subjects completed two exercise tests, 7 days apart, with saline (control, CON) or NAC infusion before and during exercise. Exercise tests comprised of cycling at 71% VO(2peak) for 45 min, then 92% VO(2peak) to fatigue, with vastus lateralis biopsies at pre-infusion, after 45 min cycling and at fatigue. Results:  Analysis was conducted on the mitogen-activated protein kinase signalling pathways, demonstrating that NAC infusion blocked the exercise-induced increase in JNK phosphorylation, but not ERK1/2, or p38 MAPK. Nuclear factor-κB p65 phosphorylation was unaffected by exercise; however it was reduced in NAC at fatigue by 14% (P<0.05) compared to pre-infusion. Analysis of exercise and/or ROS sensitive genes demonstrated that exercise-induced mRNA expression is ROS dependent for MnSOD, but not PGC-1α, interleukin-6, monocyte chemotactic protein-1, or heat-shock protein 70. Conclusion:  These results suggest that inhibition of ROS attenuates some skeletal muscle cell signalling pathways and gene expression involved in adaptations to exercise.
A C Petersen; M J McKenna; I Medved; K T Murphy; M J Brown; P Della Gatta; D Cameron-Smith
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2011-8-9
Journal Detail:
Title:  Acta physiologica (Oxford, England)     Volume:  -     ISSN:  1748-1716     ISO Abbreviation:  -     Publication Date:  2011 Aug 
Date Detail:
Created Date:  2011-8-10     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101262545     Medline TA:  Acta Physiol (Oxf)     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2011 Scandinavian Physiological Society.
School of Exercise and Nutrition Sciences, Deakin University, Melbourne, Victoria, Australia. School of Sport and Exercise Science, Victoria University, Melbourne, Victoria, Australia Institute of Sport, Exercise and Active Living, Victoria University, Melbourne, Victoria, Australia Department of Anaesthesia, Austin and Repatriation Medical Centre, Melbourne, Victoria, Australia.
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