| Infrared spectroscopic studies of solvent-induced conformational changes in globular proteins. | |
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MedLine Citation:
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PMID: 1148218 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Infrared absorption spectroscopy has been used to study the effect of organic solvents on the conformation of myoglobin, apomyoglobin, hemoglobin, lysozyme and ribonuclease. Beta structure can easily be induced by specific solvent effects. Films prepared from a 50% (v/v) mixture of alcohol, acetone, pyridine, tetrahydrofuran or dimethylsulfoxide/water mixtures show a high proportion of beta structure. The degree of induction of beta structure depends on the hydrocarbon content of the alcohol in the order methanol greater than ethanol greater than butanol. No beta structure was observed in films prepared from aqueous octanol solutions. Lyophilization tends to decrease secondary structure. The conformation of the proteins depends on the particular solvent system and the solvent composition. Solution studies of myoglobin in pure dimethylsulfoxide show that the conformation is a mixture of random and beta forms while in dimethylsulfoxide/2H2O mixtures the conformation is a mixture of alpha-helical and beta forms. |
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Authors:
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A L Jacobson; P J Krueger |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Biochimica et biophysica acta Volume: 393 ISSN: 0006-3002 ISO Abbreviation: Biochim. Biophys. Acta Publication Date: 1975 Jun |
Date Detail:
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Created Date: 1975-10-28 Completed Date: 1975-10-28 Revised Date: 2000-12-18 |
Medline Journal Info:
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Nlm Unique ID: 0217513 Medline TA: Biochim Biophys Acta Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 274-83 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Acetone
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pharmacology Alcohols / pharmacology Apoproteins Deuterium / pharmacology Dimethyl Sulfoxide / pharmacology Freeze Drying Furans / pharmacology Globulins* Hemoglobins Hydrocarbons / pharmacology Muramidase Myoglobin Protein Conformation / drug effects Pyridines / pharmacology Ribonucleases Serum Albumin, Bovine Solvents Spectrophotometry, Infrared |
| Chemical | |
Reg. No./Substance:
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0/Alcohols; 0/Apoproteins; 0/Furans; 0/Globulins; 0/Hemoglobins; 0/Hydrocarbons; 0/Myoglobin; 0/Pyridines; 0/Serum Albumin, Bovine; 0/Solvents; 67-64-1/Acetone; 67-68-5/Dimethyl Sulfoxide; 7782-39-0/Deuterium; EC 3.1.-/Ribonucleases; EC 3.2.1.17/Muramidase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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