Document Detail


Infrared spectra and molar absorption coefficients of the 20 alpha amino acids in aqueous solutions in the spectral range from 1800 to 500 cm(-1).
MedLine Citation:
PMID:  16458063     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In this work, we present the absorption spectra and molar coefficients of all 20 amino acids in aqueous solutions down to 500 cm(-1). The spectral region between 1200 and 500 cm(-1) was yet disregarded for protein infrared spectroscopy, mainly due to the strong H(2)O absorption. Absorption spectra were obtained mainly for physiological relevant pH region. Intense bands for aromatic amino acids, histidine and such with OH group could clearly be identified throughout the given spectral region. For sulfur-containing amino acids cysteine and methionine some strong bands besides the weak carbon-sulfur stretching vibration was shown. Effects of aqueous solution environment, pH, protonation states were discussed, together with previously reported data from theoretical approaches. With this complete set of spectral information application to proteins in the whole mid infrared region could be described precise and the potential of the lower spectral region to study typical cofactor ligands like histidine, shown.
Authors:
Martina Wolpert; Petra Hellwig
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2006-02-07
Journal Detail:
Title:  Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy     Volume:  64     ISSN:  1386-1425     ISO Abbreviation:  Spectrochim Acta A Mol Biomol Spectrosc     Publication Date:  2006 Jul 
Date Detail:
Created Date:  2006-06-09     Completed Date:  2006-09-08     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9602533     Medline TA:  Spectrochim Acta A Mol Biomol Spectrosc     Country:  England    
Other Details:
Languages:  eng     Pagination:  987-1001     Citation Subset:  IM    
Affiliation:
Institut für Biophysik, Johann Wolfgang Goethe-Universität, Max-von-Laue-Strasse 1, 60438 Frankfurt/Main, Germany.
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MeSH Terms
Descriptor/Qualifier:
Absorption
Amino Acids / analysis*,  chemistry
Carbon / chemistry
Cysteine / chemistry
Histidine / chemistry
Hydrogen-Ion Concentration
Methionine / chemistry
Protons
Spectrophotometry
Spectrophotometry, Infrared / methods*
Spectroscopy, Fourier Transform Infrared / methods*
Sulfur / chemistry
Water / chemistry
Chemical
Reg. No./Substance:
0/Amino Acids; 0/Protons; 52-90-4/Cysteine; 63-68-3/Methionine; 71-00-1/Histidine; 7440-44-0/Carbon; 7704-34-9/Sulfur; 7732-18-5/Water

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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