Document Detail


Infrared microspectroscopy of live cells in aqueous media.
MedLine Citation:
PMID:  20981390     Owner:  NLM     Status:  In-Process    
Abstract/OtherAbstract:
Fourier Transform Infrared (FTIR) spectroscopic measurements of individual, live HeLa cells in culture and buffer media are presented. Spectral data were acquired using a newly designed live cell chamber developed in the authors' laboratory. Data were processed using MATLAB-based routines that correct for the overcompensation of water encountered during live cell measurements in aqueous samples. Data presented are from live cells monitored over an extended period of time as well as a comparison of live cells exposed to perturbing conditions.
Authors:
Ellen J Swain Marcsisin; Christina M Uttero; Miloš Miljković; Max Diem
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2010-10-28
Journal Detail:
Title:  The Analyst     Volume:  135     ISSN:  1364-5528     ISO Abbreviation:  Analyst     Publication Date:  2010 Dec 
Date Detail:
Created Date:  2010-11-18     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0372652     Medline TA:  Analyst     Country:  England    
Other Details:
Languages:  eng     Pagination:  3227-32     Citation Subset:  IM    
Affiliation:
Laboratory for Spectral Diagnosis, Department of Chemistry and Chemical Biology, Northeastern University, Boston, MA 02115, USA.
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MeSH Terms
Descriptor/Qualifier:
Grant Support
ID/Acronym/Agency:
CA 090346/CA/NCI NIH HHS

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