| Infrared light induced patterning of proteins on ppNIPAM thermoresponsive thin films: a "protein laser printer". | |
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MedLine Citation:
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PMID: 20358117 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Protein micropatterns have applications in fundamental life sciences and clinical medicine. In this work, we present a new technique to create 2-D protein micropatterns by local activation of a thin film of thermoresponsive plasma-deposited poly(N-isopropylacrylamide) (ppNIPAM) using a computer-controlled infrared laser beam. While the whole substrate is exposed to the protein solution, protein deposition happens only at laser-activated locations. A few seconds of laser exposure is all that is required to form a pattern with resolution in the single micrometre range. Successful ligand binding after protein deposition indicates that protein function remains intact after laser-induced adsorption onto ppNIPAM. This rapid, simple technique advances currently available strategies for protein patterning by its potential to pattern proteins in an enclosed environment or onto a 3-D scaffold. |
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Authors:
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Xuanhong Cheng; E Yegan Erdem; Shoji Takeuchi; Hiroyuki Fujita; Buddy D Ratner; Karl F B?hringer |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, Non-P.H.S. Date: 2010-01-20 |
Journal Detail:
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Title: Lab on a chip Volume: 10 ISSN: 1473-0197 ISO Abbreviation: Lab Chip Publication Date: 2010 Apr |
Date Detail:
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Created Date: 2010-04-01 Completed Date: 2010-06-28 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101128948 Medline TA: Lab Chip Country: England |
Other Details:
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Languages: eng Pagination: 1079-85 Citation Subset: IM |
Affiliation:
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Materials Science and Engineering, Bioengineering, Lehigh University, Bethlehem, PA 18015, USA. xuc207@lehigh.edu |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Acrylamides
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chemistry* Adsorption Equipment Design Equipment Failure Analysis Lasers Membranes, Artificial* Microfluidic Analytical Techniques / instrumentation* Micromanipulation / instrumentation* Printing Protein Array Analysis / instrumentation* Protein Binding Proteins / chemistry* Temperature |
| Grant Support | |
ID/Acronym/Agency:
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1-P50-HG002360-01/HG/NHGRI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Acrylamides; 0/Membranes, Artificial; 0/Proteins; 2210-25-5/N-isopropylacrylamide |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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