Document Detail


Infrared light induced patterning of proteins on ppNIPAM thermoresponsive thin films: a "protein laser printer".
MedLine Citation:
PMID:  20358117     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Protein micropatterns have applications in fundamental life sciences and clinical medicine. In this work, we present a new technique to create 2-D protein micropatterns by local activation of a thin film of thermoresponsive plasma-deposited poly(N-isopropylacrylamide) (ppNIPAM) using a computer-controlled infrared laser beam. While the whole substrate is exposed to the protein solution, protein deposition happens only at laser-activated locations. A few seconds of laser exposure is all that is required to form a pattern with resolution in the single micrometre range. Successful ligand binding after protein deposition indicates that protein function remains intact after laser-induced adsorption onto ppNIPAM. This rapid, simple technique advances currently available strategies for protein patterning by its potential to pattern proteins in an enclosed environment or onto a 3-D scaffold.
Authors:
Xuanhong Cheng; E Yegan Erdem; Shoji Takeuchi; Hiroyuki Fujita; Buddy D Ratner; Karl F B?hringer
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2010-01-20
Journal Detail:
Title:  Lab on a chip     Volume:  10     ISSN:  1473-0197     ISO Abbreviation:  Lab Chip     Publication Date:  2010 Apr 
Date Detail:
Created Date:  2010-04-01     Completed Date:  2010-06-28     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101128948     Medline TA:  Lab Chip     Country:  England    
Other Details:
Languages:  eng     Pagination:  1079-85     Citation Subset:  IM    
Affiliation:
Materials Science and Engineering, Bioengineering, Lehigh University, Bethlehem, PA 18015, USA. xuc207@lehigh.edu
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MeSH Terms
Descriptor/Qualifier:
Acrylamides / chemistry*
Adsorption
Equipment Design
Equipment Failure Analysis
Lasers
Membranes, Artificial*
Microfluidic Analytical Techniques / instrumentation*
Micromanipulation / instrumentation*
Printing
Protein Array Analysis / instrumentation*
Protein Binding
Proteins / chemistry*
Temperature
Grant Support
ID/Acronym/Agency:
1-P50-HG002360-01/HG/NHGRI NIH HHS
Chemical
Reg. No./Substance:
0/Acrylamides; 0/Membranes, Artificial; 0/Proteins; 2210-25-5/N-isopropylacrylamide

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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