Document Detail


Influenza A virus polymerase: structural insights into replication and host adaptation mechanisms.
MedLine Citation:
PMID:  20538599     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The heterotrimeric RNA-dependent RNA polymerase of influenza viruses catalyzes RNA replication and transcription activities in infected cell nuclei. The nucleotide polymerization activity is common to both replication and transcription processes, with an additional cap-snatching function being employed during transcription to steal short 5'-capped RNA primers from host mRNAs. Cap-binding, endonuclease, and polymerase activities have long been studied biochemically, but structural studies on the polymerase and its subunits have been hindered by difficulties in producing sufficient quantities of material. Recently, because of heightened effort and advances in expression and crystallization technologies, a series of high resolution structures of individual domains have been determined. These shed light on intrinsic activities of the polymerase, including cap snatching, subunit association, and nucleocytoplasmic transport, and open up the possibility of structure-guided development of new polymerase inhibitors. Furthermore, the activity of influenza polymerase is highly host- and cell type-specific, being dependent on the identity of a few key amino acid positions in the different subunits, especially in the C-terminal region of PB2. New structures demonstrate the surface exposure of these residues, consistent with ideas that they might modulate interactions with host-specific factors that enhance or restrict activity. Recent proteomic and genome-wide interactome and RNA interference screens have suggested the identities of some of these potential regulators of polymerase function.
Authors:
Stéphane Boivin; Stephen Cusack; Rob W H Ruigrok; Darren J Hart
Related Documents :
14525979 - Structure-function analysis of trypanosoma brucei rna triphosphatase and evidence for a...
8508779 - Essential nucleotide sequences and secondary structure elements of the hairpin ribozyme.
10788319 - Effects of phosphorothioate modifications on precursor trna processing by eukaryotic rn...
17524419 - Crystal structures of trna-guanine transglycosylase (tgt) in complex with novel and pot...
19634899 - Single-atom imino substitutions at a9 and a10 reveal distinct effects on the fold and f...
1742349 - How many ef-tu molecules participate in aminoacyl-trna binding?
8679649 - Interactions of alkylglucosides with the renal sodium/d-glucose cotransporter.
15797249 - Probing prime substrate binding sites of human dipeptidyl peptidase-iv using competitiv...
22030389 - Structure of collagen receptor integrin α(1)i domain carrying the activating mutation e...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Review     Date:  2010-06-10
Journal Detail:
Title:  The Journal of biological chemistry     Volume:  285     ISSN:  1083-351X     ISO Abbreviation:  J. Biol. Chem.     Publication Date:  2010 Sep 
Date Detail:
Created Date:  2010-09-06     Completed Date:  2010-10-07     Revised Date:  2011-09-13    
Medline Journal Info:
Nlm Unique ID:  2985121R     Medline TA:  J Biol Chem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  28411-7     Citation Subset:  IM    
Affiliation:
Unit of Virus Host-Cell Interactions, UMI3265, UJF-EMBL-CNRS, France.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Active Transport, Cell Nucleus
Animals
Catalytic Domain
Cell Nucleus / enzymology,  virology
Crystallography, X-Ray
Humans
Influenza A virus / enzymology*
Protein Structure, Quaternary
RNA Caps / biosynthesis
RNA, Viral / biosynthesis
RNA-Directed DNA Polymerase / chemistry*,  metabolism
Structure-Activity Relationship
Transcription, Genetic
Virus Replication / physiology
Chemical
Reg. No./Substance:
0/RNA Caps; 0/RNA, Viral; EC 2.7.7.49/RNA-Directed DNA Polymerase
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Influenza hemagglutinin and neuraminidase membrane glycoproteins.
Next Document:  Pgrmc1 (progesterone receptor membrane component 1) associates with epidermal growth factor receptor...