| Influencing electroosmotic flow and selectivity in open tubular electrochromatography by tetrakis(pentafluorophenyl)porphyrin as capillary wall modifier. | |
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MedLine Citation:
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PMID: 12685589 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A physically adsorbed and covalently bonded porphyrin derivative, 5,10,15,20-tetrakis(pentafluorophenyl)porphyrin, H2TPFPP, has been used as a fused-silica capillary wall modifier in open tubular capillary electrochromatography (OT-CEC), and its influence on the electroosmotic flow (EOF) velocity and on the selectivity of OT-CEC separations of a set of model aromatic carboxylic acids has been tested. Whereas most of the coatings of this category bring about an increase in selectivity with a concomitant slow down of the EOF, H2TPFPP coating, depending on pH of the background electrolyte used, resulted both in decreasing of EOF at pH 8.5 by 5% and in increasing of EOF by 10-43% at pH 6 and 5, respectively. The separation efficiency and the resolution of aromatic carboxylic acids separation in coated capillaries, namely in that one with covalent coating, were better than in the bare fused-silica capillary. The perspectives of H2TPFPP as capillary wall modifier are visualized in introducing well defined electroosmotic properties of materials used for miniaturized separation channels preparation in chip-based electromigration devices. |
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Authors:
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Jana Charvátová; Václav Kasicka; Zdenek Deyl; Vladimír Král |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of chromatography. A Volume: 990 ISSN: 0021-9673 ISO Abbreviation: J Chromatogr A Publication Date: 2003 Mar |
Date Detail:
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Created Date: 2003-04-10 Completed Date: 2003-11-20 Revised Date: 2009-01-15 |
Medline Journal Info:
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Nlm Unique ID: 9318488 Medline TA: J Chromatogr A Country: Netherlands |
Other Details:
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Languages: eng Pagination: 111-9 Citation Subset: IM |
Affiliation:
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Institute of Chemical Technology, Technická 5, 166 28 Prague 6, Czech Republic. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Chromatography, Micellar Electrokinetic Capillary
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methods* Hydrogen-Ion Concentration Osmosis Porphyrins / chemistry* |
| Chemical | |
Reg. No./Substance:
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0/Porphyrins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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