Document Detail


Influences of cyclooxygenase-1 and -2 expression on the radiosensitivities of human cervical cancer cell lines.
MedLine Citation:
PMID:  17601662     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We utilized three cervical cancer cell lines (HeLa, HT-3, and C33A) and clonogenic assays to determine whether cyclooxygenase (COX) expression is related to radiosensitivity. Using COX DNA transfection and COX inhibition by siRNA, we also examined changes in radiosensitivity caused by variations in COX expression. The survival fractions of HeLa and HT-3 cell lines, which both with COX-1 and COX-2 activity, were found to be significantly higher than that of the C33A cell line which had neither COX-1 nor COX-2 activity. Moreover, the acquisition of COX-1 in C33A cell line significantly reduced its radiosensitivity, but COX-2 transfection increased radiosensitivity in this cell line. In addition, the inhibition of COX-1 activity in HT-3 cell line using siRNA resulted in an increased radiosensitivity, but this phenomenon was not observed for COX-2 inhibition. The same experiment in HeLa cells using siRNA also showed no significant change in radiosensitivity. The results obtained during this study suggest that COX expression is associated with the radiosensitivity in uterine cervical cancer cell lines and COX-1 might have more important role than COX-2.
Authors:
Yong-Tark Jeon; Yoo-Cheol Song; Su-Hyeong Kim; Hong-Gyun Wu; Il-Han Kim; In-Ae Park; Jae Weon Kim; Noh-Hyun Park; Soon-Beom Kang; Hyo-Pyo Lee; Yong-Sang Song
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2007-06-29
Journal Detail:
Title:  Cancer letters     Volume:  256     ISSN:  0304-3835     ISO Abbreviation:  Cancer Lett.     Publication Date:  2007 Oct 
Date Detail:
Created Date:  2007-09-05     Completed Date:  2007-11-06     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7600053     Medline TA:  Cancer Lett     Country:  Ireland    
Other Details:
Languages:  eng     Pagination:  33-8     Citation Subset:  IM    
Affiliation:
Department of Obstetrics and Gynecology, College of Medicine, Seoul National University, 28 Yungun-Dong, Jongno-Gu, 110-744 Seoul, Republic of Korea.
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MeSH Terms
Descriptor/Qualifier:
Blotting, Western
Cesium Radioisotopes
Colony-Forming Units Assay
Cyclooxygenase 1 / physiology*
Cyclooxygenase 2 / physiology*
Female
Gamma Rays
Humans
RNA, Small Interfering / pharmacology
Radiation Tolerance*
Tumor Cells, Cultured / enzymology,  radiation effects
Uterine Cervical Neoplasms / enzymology,  radiotherapy*
Chemical
Reg. No./Substance:
0/Cesium Radioisotopes; 0/RNA, Small Interfering; EC 1.14.99.1/Cyclooxygenase 1; EC 1.14.99.1/Cyclooxygenase 2

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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