| Influence of vitamins and osmolites on growth and bacteriocin production by Lactobacillus salivarius CRL 1328 in a chemically defined medium. | |
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MedLine Citation:
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PMID: 19370073 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The aim of this study was to analyze the influence of vitamins, glycerol, and salts on the growth and bacteriocin production by Lactobacillus salivarius CRL 1328, a human vagina isolate, by using a chemically defined medium to determine the optimal conditions for salivaricin production. The single omission of <span class="smallcap">d</span>-biotin, thiamine, p-aminobenzoic acid, folic acid, or cyanocobalamin did not affect the bacterial growth, whereas the removal of nicotinic acid, riboflavin, and pyridoxal produced a decrease of about 30% in the growth rate. Maximum salivaricin activity was observed after the addition of 5 or 10 g/L of NaCl. On the basis of the nutritional requirements and the levels of salivaricin production, a new optimized and simplified defined medium (SDM-NaCl) for L. salivarius CRL 1328 bacteriocin production was formulated. The kinetics of salivaricin production in SDM-NaCl and in the complex media LAPTg revealed that bacteriocin production was growth linked. A combination of tricine - sodium dodecyl sulfate polyacrylamide gel electrophoresis (Tricine-SDS-PAGE), Lumitein protein gel staining, and a bioassay for antibacterial activity indicated that the molecular mass of salivaricin CRL 1328 is about 4.5 kDa. The partially purified bacteriocin, obtained from SDM-NaCl after concentration, allowed for the design of a relatively simple method for the recovery of a biologically active protein. |
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Authors:
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Esteban Vera Pingitore; Elvira María Hebert; Fernando Sesma; María Elena Nader-Macías |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Canadian journal of microbiology Volume: 55 ISSN: 0008-4166 ISO Abbreviation: Can. J. Microbiol. Publication Date: 2009 Mar |
Date Detail:
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Created Date: 2009-04-16 Completed Date: 2009-07-29 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0372707 Medline TA: Can J Microbiol Country: Canada |
Other Details:
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Languages: eng Pagination: 304-10 Citation Subset: IM |
Affiliation:
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Centro de Referencia para Lactobacilos (CERELA-CONICET), Chacabuco 145 (T4000ILC), San Miguel de Tucuman 4000, Argentina. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Bacteriocins
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biosynthesis*,
pharmacology Culture Media* Enterococcus faecalis / drug effects, growth & development Female Glycerol / metabolism, pharmacology* Humans Lactobacillus / drug effects, growth & development*, isolation & purification, metabolism* Microbial Sensitivity Tests Osmotic Pressure Sodium Chloride / metabolism, pharmacology* Vagina / microbiology Vitamins / metabolism, pharmacology* |
| Chemical | |
Reg. No./Substance:
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0/Bacteriocins; 0/Culture Media; 0/Vitamins; 56-81-5/Glycerol; 7647-14-5/Sodium Chloride |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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