Document Detail


Influence of vitamin A palmitate on the growth of regenerating Triturus alpestris forelimbs.
MedLine Citation:
PMID:  3196784     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The objective of this investigation was to analyse the effects of vitamin A palmitate orally administered to young postmetamorphic Triturus alpestris on the growth of regenerates. Vitamin A was administered for 4, 7, 11 and 14 days after amputation at the middle of the right zeugopod. Growth of regenerates was evaluated on the basis of external size measurements and blastema cell proliferation. Calculation of the percentages of cells in each cell-cycle phase and estimation of mitotic indices during the period of drug administration were performed by microdensitometric scanning of Feulgen-stained nuclei and counting of chromosome configurations, respectively. Vitamin A was found to induce polyploidism, to enhance transition from G1- to S-phase cell nuceli, to prolong the duration of G2-phase to the detriment of S-phase and to promote mitotic activity in the mesenchyme cells. In accordance with the above, an acceleration in the growth rates of treated blastemas with respect to controls was observed.
Authors:
S Koussoulakos; H J Anton
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Biological structures and morphogenesis     Volume:  1     ISSN:  0989-8972     ISO Abbreviation:  Biol Struct Morphog     Publication Date:  1988  
Date Detail:
Created Date:  1989-01-17     Completed Date:  1989-01-17     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  8807439     Medline TA:  Biol Struct Morphog     Country:  FRANCE    
Other Details:
Languages:  eng     Pagination:  124-9     Citation Subset:  IM    
Affiliation:
University of Athens, Zoological Laboratory, Greece.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Division
Forelimb / cytology,  physiology*
Regeneration / drug effects*
Time Factors
Triturus / physiology*
Vitamin A / analogs & derivatives*,  pharmacology
Chemical
Reg. No./Substance:
11103-57-4/Vitamin A; 79-81-2/retinol palmitate

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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