| Influence of vitamin A on matrix-induced endochondral bone formation. | |
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MedLine Citation:
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PMID: 6652548 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The influence of retinoic acid on matrix-induced endochondral bone differentiation was determined. Retinoic acid was administered during discrete stages of endochondral bone formation, specifically, mesenchymal cell proliferation, chondrogenesis, bone formation, and mineralization. In retinoic acid-treated rats examined on day 3 following matrix implantation, biochemical markers for mesenchymal cell proliferation were about 50% of the controls. Chondrogenesis on day 7, assessed by 35 SO4 incorporation into proteoglycans, was 27% of the control. In addition, dissociative extraction of proteoglycans with 4.0 M guanidine-HCl and chromatography on Sepharose CL-2B revealed the synthesis of a smaller molecular weight proteoglycan when compared to controls which exhibited the cartilage-specific type. Osteogenesis and bone mineralization were monitored by alkaline phosphatase activity and 45Ca incorporation. On day 11 alkaline phosphatase activity was decreased by 40% and 45Ca incorporation was 48% of the control. These results revealed the multiple foci of the actions of excess vitamin A. |
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Authors:
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D P DeSimone; A H Reddi |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Calcified tissue international Volume: 35 ISSN: 0171-967X ISO Abbreviation: Calcif. Tissue Int. Publication Date: 1983 Sep |
Date Detail:
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Created Date: 1984-02-24 Completed Date: 1984-02-24 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 7905481 Medline TA: Calcif Tissue Int Country: GERMANY, WEST |
Other Details:
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Languages: eng Pagination: 732-9 Citation Subset: IM; S |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Alkaline Phosphatase
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metabolism Animals Bone Matrix / cytology*, metabolism Cartilage / cytology*, metabolism Cell Division / drug effects Ornithine Decarboxylase / metabolism Osteoblasts / cytology* Osteogenesis / drug effects* Proteoglycans / metabolism Rats Tretinoin / pharmacology* |
| Grant Support | |
ID/Acronym/Agency:
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F32 AMO6547-01/AM/NIADDK NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Proteoglycans; 302-79-4/Tretinoin; EC 3.1.3.1/Alkaline Phosphatase; EC 4.1.1.17/Ornithine Decarboxylase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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