| Influence of various fatty acids on the activity of protein phosphatase type 2C and apoptosis of endothelial cells and macrophages. | |
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MedLine Citation:
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PMID: 18812221 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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In previous work we have demonstrated that protein phosphatase type 2C (PP2C) alpha and beta can be activated by mono-unsaturated fatty acids (MUFAs) leading to apoptosis of cultured endothelial cells. In the present paper we could show that saturated fatty acids (SFAs) did not activate PP2C and did not cause apoptosis both in endothelial cells and macrophages. However, long-chain SFAs (>16 C-atoms) were capable of inhibiting both, activation of PP2C as well as apoptosis of human umbilical vein endothelial cells (HUVECs) and macrophages caused by oleic acid. Interestingly, docosahexaenoic acid (DHA) known to protect arterial vessels against the progression of atherosclerosis caused apoptosis of HUVECs at high concentrations (200-400microM) but inhibited the apoptotic damage of HUVECs at a low, physiologically relevant concentration range (1-10microM). In contrast, oleic acid did not protect HUVECs against damage even at low concentrations (1-25microM). It is supposed that an unbalanced and chronically increased level of MUFAs in blood has an atherosclerotic potential. Furthermore, PP2C activated by MUFAs appears as a new target for drugs to prevent or treat atherosclerosis. |
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Authors:
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Josef Krieglstein; Birgit Hufnagel; Melanie Dworak; Stefanie Schwarz; Tobias Kewitz; Michael Reinbold; Susanne Klumpp |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2008-09-04 |
Journal Detail:
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Title: European journal of pharmaceutical sciences : official journal of the European Federation for Pharmaceutical Sciences Volume: 35 ISSN: 0928-0987 ISO Abbreviation: Eur J Pharm Sci Publication Date: 2008 Dec |
Date Detail:
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Created Date: 2008-11-21 Completed Date: 2009-02-20 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9317982 Medline TA: Eur J Pharm Sci Country: Netherlands |
Other Details:
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Languages: eng Pagination: 397-403 Citation Subset: IM |
Affiliation:
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Institut für Pharmakologie und Toxikologie, Philipps-Universitaet, Marburg, Germany. krieglst@uni-muenster.de |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Apoptosis
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drug effects,
physiology* Bisbenzimidazole Docosahexaenoic Acids / pharmacology Endothelial Cells / drug effects*, enzymology* Fatty Acids / pharmacology* Fluorescent Dyes Humans Macrophages / drug effects*, enzymology* Oleic Acid / pharmacology Oxazines Phosphoprotein Phosphatases / metabolism* |
| Chemical | |
Reg. No./Substance:
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0/Fatty Acids; 0/Fluorescent Dyes; 0/Oxazines; 112-80-1/Oleic Acid; 23491-44-3/Bisbenzimidazole; 2381-85-3/Nile Blue; 25167-62-8/Docosahexaenoic Acids; EC 3.1.3.16/Phosphoprotein Phosphatases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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