Document Detail


Influence of the uterine environment on the development of in vitro produced equine embryos.
MedLine Citation:
PMID:  22089531     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
The necessity for early interaction between the embryo and the oviductal and/or uterine environment in the horse is reflected by several striking differences between equine embryos that develop in vivo and those produced in vitro. Better understanding of the salient interactions may help to improve the efficiency of in vitro equine embryo production. In an initial experiment, cleavage-stage in vitro produced (IVP) equine embryos were transferred into the uterus of recipient mares that had ovulated recently to determine whether premature placement in this in vivo environment would improve subsequent development. In a second experiment, an important element of the uterine environment was mimicked by adding uterocalin, a major component of the endometrial secretions during early pregnancy, to the culture medium. Intra-uterine transfer of cleavage-stage equine IVP embryos yielded neither ultrasonographically detectable pregnancies nor day 7 blastocysts, indicating that the uterus is not a suitable environment for pre-compact morula stage horse embryos. By contrast, exposure to uterocalin during IVP improved capsule formation, although it did not measurably affect development or expression of a panel of genes known to differ between in vivo and in vitro embryos. Further studies are required to evaluate whether uterocalin serves purely as a carrier protein or more directly promotes improved capsule development.
Authors:
Katrien Smits; Jan Govaere; Luc J Peelman; Karen Goossens; Dirk C de Graaf; Dries Vercauteren; Leen Vandaele; Maarten Hoogewijs; Eline Wydooghe; Tom Arjun Edgar Stout; Ann Van Soom
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2011-11-16
Journal Detail:
Title:  Reproduction (Cambridge, England)     Volume:  -     ISSN:  1741-7899     ISO Abbreviation:  -     Publication Date:  2011 Nov 
Date Detail:
Created Date:  2011-11-17     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100966036     Medline TA:  Reproduction     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
K Smits, Reproduction, Obstetrics and Herd Health, Ghent University, Faculty of Veterinary Medicine, Merelbeke, Belgium.
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